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AB065. 229. On the growth and form of the mesentery

Kevin G VSports. Byrnes1,2, Dara Walsh1,2, M. Fahad Ullah1, Omer Hashmi1, Daniel Westby1, Anna Garritt3, Rosa Mirapeix3, Wout Lamers4,5, Yi Wu7, S. Xiang Zhang7, Fabio Quondamatteo8, Peter Dockery8, Kieran W. McDermott2, J. Calvin Coffey1,2,8; Human Mesentery Project Consortium.

1University Hospital Limerick, Limerick, Ireland;2Department of Anatomy, Graduate Entry Medical School, University of Limerick, Limerick, Ireland;3Department of Embryology, Universitat Autònoma de Barcelona, Barcelona, Spain;4Department of Anatomy & Embryology, Maastricht University, Maastricht, the Netherlands;5Tygat Institute for Liver and Intestinal Research, Academic Medical Center, Amsterdam, Netherlands;6Department of Anatomy and Embryology, Chongqing Medical University, Chongqing 400016, China;7Department of Anatomy, National University of Ireland, Galway, Ireland;84i Centre for Interventions in Infection, Inflammation & Immunity, University of Limerick, Limerick, Ireland

Background: Systematic study of the mesentery has demonstrated that it is both continuous and spiral in nature from duodenum to rectum. Embryological planes are commonly exploited during abdominal surgery; however, the exact morphogenesis of the mesentery remains unknown. This study aimed to characterise the three-dimensional structure of the developing mesentery V体育官网.

Methods: Embryological (n=12), foetal (n=4) and cadaveric (n=2) specimens were sectioned, stained and digitized. Regression analysis (SIFT; ImageJ2, v1. 50e, NIH, USA) stacked sections in their true alignment VSports手机版. An internal panel of two reviewers verified manual tracings of regional anatomy and performed a double-blinded comparison of age-matched embryos. To test the reliability of manual tracing, five operators independently traced developing structures. Findings from three-dimensional outputs were further investigated with cadaveric dissection.

Results: Areas of mesoderm, endoderm and vasculature were reliably traced by operators [intra-class correlation coefficients 0. 999 (95% CI, 0. 998–0. 999), 0. 976 (95% CI, 0. 938–0. 994), and 0. 995 (95% CI, 0. 988–0. 999) respectively]. Rendered volumes had a high degree of spatial overlap between operators [Sørensen-Dice similarity coefficient (mean ± SD) of mesoderm 0. 9949±0. 0085 (0. 994–0. 996), endoderm 0. 9224±0. 0067 (0. 91–0. 931), and vasculature 0. 9379±0. 00365 (0 V体育安卓版. 933–0. 944)]. Bland-Altman analysis demonstrated no systematic bias between operators. Three-dimensional visualisation of fore-, mid- and hindgut regions of the mesentery enabled identification of continuity between all regions in every specimen. Cadaveric dissection replicated three-dimensional observations.

Conclusions: The mesentery, continuous from mesogastric to mesorectal level, is the exclusive intermediate between viscera and the remainder of the body V体育ios版. This developmental model provides a structural framework for understanding and further investigating visceral and neurovascular development.

Keywords: Mesentery; embryology; development

doi: 10.21037/map.2018.AB065

Cite this abstract as: Byrnes KG, Walsh D, Ullah MF, Hashmi O, Westby D, Garritt A, Mirapeix R, Lamers W, Wu Y, Zhang SX, Quondamatteo F, Dockery P, McDermott KW, Coffey JC; Human Mesentery Project Consortium. On the growth and form of the mesentery. Mesentery Peritoneum 2018;2:AB065. doi: 10.21037/map.2018.AB065

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