"V体育安卓版" Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely V体育官网. .

. 2025 Sep 15:2025:8574340.
doi: 10.1155/mi/8574340. eCollection 2025.

The Long Noncoding RNA, LOC645166, in T Cells of Ankylosing Spondylitis (AS) Patients Regulates the FOXP3 Expression via the Axis of LOC645166/miR-188-5p/NFKBID

Hui-Chun Yu  1 , Kuang-Yung Huang  2   3 , Ming-Chi Lu  1   2   3 , Hsien-Yu Huang Tseng  1 , Ning-Sheng Lai  2   3 , Hsien-Bin Huang  4
Affiliations

The Long Noncoding RNA, LOC645166, in T Cells of Ankylosing Spondylitis (AS) Patients Regulates the FOXP3 Expression via the Axis of LOC645166/miR-188-5p/NFKBID

Hui-Chun Yu et al. Mediators Inflamm. .

Abstract

The expression of long noncoding RNA (LncRNA), LOC645166, is downregulated in T cells of ankylosing spondylitis (AS) patients. The role of LOC645166 in contribution to AS pathogenesis was investigated. Here, we have identified that an interacting network of LOC645166/miR-188-5p/NF-κB inhibitor-D (NFKBID) occurs in T cells of AS patients and regulates the expression of forkhead box P3 (FOXP3). Downregulation of LOC645166 augments the levels of miR-188-5p that binds to the 3'-UTR of NFKB1D mRNA and blocks the NFKB1D expression. NFKB1D, also called IκBNS, can trigger regulatory T (Treg) cell development through induction of FOXP3 expression VSports手机版. Downregulation of NFKB1D expression leads to suppression of the FOXP3 induction, in turn affecting Treg cell development. Promotion of the autoimmune response induced by suppression of FOXP3 expression contributes to the pathogenesis of AS. .

PubMed Disclaimer

Conflict of interest statement (VSports)

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
The LOC645166/miR-188-5p/NFKBID interaction network is present in T cells of AS patients. (A) LOC645166 hybridizes with miR-188-5p. (B) miR-188-5p hybridizes with the 3′-UTR of NFKBID mRNA. (C) The effect of miR-188-5p overexpressed in Jurkat cells on LOC645166 and NFKBID mRNA coimmunoprecipitated by anti-Ago1/2/3 antibodies (N = 4). (D) The expression levels of miR-188-5p in T cells of AS patients and healthy controls (N = 13). (E) The analysis of NFKBID protein in T cells of AS patients and healthy controls by the western blotting (N = 5).
Figure 2
Figure 2
The effect of LOC645166 overexpressed in Jurkat cells on the levels of miR-188-5p and NFKBID protein. (A) The effect of LOC645166 overexpressed in Jurkat cells on the levels of miR-188-5p (N = 4). (B) The effect of LOC645166 overexpressed in Jurkat cells on the protein levels of NFKBID (N = 4). (C) The effect of miR-188-5p overexpressed in Jurkat cells on the protein levels of NFKBID. (D) The effect of LOC645166 co-overexpressed with miR-188-5p in Jurkat cells on the protein levels of NFKBID (N = 4).
Figure 3
Figure 3
The effect of miR-188-5p inhibitor electro-transferred in Jurkat cells on the levels of miR-188-5p and of NFKBID protein. (A) The effect of miR-188-5p inhibitor electro-transferred in Jurkat cells on the levels of miR-188-5p (N = 4). (B) The effect of miR-188-5p inhibitor electro-transferred in Jurkat cells on the protein levels of NFKBID (N = 4).
Figure 4
Figure 4
The effect of LOC645166/miR-188-5p/NFKBID axis on the protein levels of FOXP3 in Jurkat cells. (A) The effect of LOC645166 overexpressed in Jurkat cells on the protein levels FOXP3 (N = 4). (B) The effect of miR-188-5p electro-transferred in Jurkat cells on the FOXP3 expression (N = 4). (C) The effect of miR-188-5p co-transfected with its inhibitor in Jurkat cells on the protein levels of FOXP3 (N = 4). (D) The effect of NFKBID overexpressed in Jurkat cells on protein levels of FOXP3 (N = 4). (E) The effect of NFKBID knocked down by siRNA on the protein levels of FOXP3 (N = 4).
Figure 5
Figure 5
The expression levels of FOXP3 and Treg cell frequencies in CD4+ T cells of AS patients and healthy control. (A) The levels of FOXP3 in CD4+ T cells of AS patients and healthy controls (N = 5). (B) Flow cytometry analysis of the Treg cell frequencies in CD4+ T cells of AS patients (N = 14) and healthy controls (N = 13).
See this image and copyright information in PMC

References

    1. Colbert R. A., DeLay M. L., Klenk E. I., Layh-Schmitt G. From HLA-B27 to Spondyloarthritis: A Journey Through the ER. Immunological Reviews . 2010;233(1):181–202. doi: 10.1111/j.0105-2896.2009.00865.x. - DOI - PMC - PubMed
    1. Melis L., Elewaut D. Progress in Spondylarthritis. Immunopathogenesis of Spondyloarthritis: Which Cells Drive Disease? Arthritis Research & Therapy . 2009;11(3) doi: 10.1186/ar2722.233 - V体育官网入口 - DOI - PMC - PubMed
    1. Smith J. A., Märker-Hermann E., Colbert R. A. Pathogenesis of Ankylosing Spondylitis: Current Concepts. Best Practice & Research Clinical Rheumatology . 2006;20(3):571–591. doi: 10.1016/j.berh.2006.03.001. - DOI - PubMed
    1. Tam L.-S., Gu J., Yu D. Pathogenesis of Ankylosing Spondylitis. Nature Reviews Rheumatology . 2010;6(7):399–405. doi: 10.1038/nrrheum.2010.79. - DOI - PubMed
    1. Caffrey M. F., James D. C. Human Lymphocyte Antigen Association in Ankylosing Spondylitis. Nature . 1973;242(5393) doi: 10.1038/242121a0.121 - DOI - PubMed

MeSH terms

VSports最新版本 - LinkOut - more resources