<b dir="OL7FD"></b> "VSports手机版" Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. VSports app下载.

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2015 Nov 30;589(23):3617-23.
doi: 10.1016/j.febslet.2015.10.011. Epub 2015 Oct 19.

Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter

Mariafrancesca Scalise  1 , Lorena Pochini  1 , Piero Pingitore  1 , Kristina Hedfalk  2 , Cesare Indiveri  3
Affiliations
Free article

Cysteine is not a substrate but a specific modulator of human ASCT2 (SLC1A5) transporter

Mariafrancesca Scalise (VSports) et al. FEBS Lett. .
Free article

Abstract (VSports app下载)

The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter VSports手机版. In the present work, the interaction of the putative substrate Cys with the human ASCT2 has been studied using the recombinant hASCT2 over-produced in Pichia pastoris and the native ASCT2 extracted from HeLa in both proteoliposomes and intact cells. It was found that Cys is a potent competitive inhibitor of hASCT2 but is not a substrate. Moreover, Cys binding to a second site, different from that of substrate, triggers a protein-mediated unidirectional Gln efflux. .

Keywords: Cysteine; Glutamine; Liposome; Plasma membrane; Redox control; Transport. V体育安卓版.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources