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. 2015 Apr 2:13:108.
doi: 10.1186/s12967-015-0468-2.

Fourier transform infrared spectroscopy for the distinction of MCF-7 cells treated with different concentrations of 5-fluorouracil (V体育官网入口)

Bi-Bo Wu  1 , Yi-Ping Gong  2 , Xin-Hong Wu  3 , Yuan-Yuan Chen  4 , Fang-Fang Chen  5 , Li-Ting Jin  6 , Bo-Ran Cheng  7 , Fen Hu  8 , Bin Xiong  9
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"VSports手机版" Fourier transform infrared spectroscopy for the distinction of MCF-7 cells treated with different concentrations of 5-fluorouracil

Bi-Bo Wu et al. J Transl Med. .

Abstract

Background: In order to provide personalized treatment to patients with breast cancer, an accurate, reliable and cost-efficient analytical technique is needed for drug screening and evaluation of tumor response to chemotherapy VSports手机版. .

Methods: Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was used as a tool to assess cancer cell response to chemotherapy. MCF-7 cells (human breast adenocarcinoma cell line) were treated with different concentrations of 5-fluorouracil (5-FU) V体育安卓版. The inhibition of cell proliferation was monitored by MTT, and apoptosis rates were determined by flow cytometry. Finally, spectra of the cell populations were acquired by ATR-FTIR. .

Results: The cell response to 5-FU was detectable at different concentrations by ATR-FTIR. First, a band observed at 1741 cm(-1), representing membrane phospholipids, was enhanced with increasing 5-FU concentrations. In addition, the MCF-7 cell spectrum shifted progressively from 1153 to 1170 cm(-1) with increasing drug doses V体育ios版. Finally, the normalized band intensity of 1741 cm(-1)/Amide I was highly correlated with the percentage of apoptotic cells as assessed by partial correlation analysis. .

Conclusions: These findings suggest that the effects of different concentrations of drugs can be monitored by ATR-FTIR, which may help evaluate the response to chemotherapy and improve treatment strategies VSports最新版本. .

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Figures

Figure 1
Figure 1
5-fluorouracil mediates in vitro cytostatic activity. Growth inhibitory effect of 5-fluorouracil on MCF-7 cells treated for 72 hours. Values represent the mean ± SEM of three independent experiments.
Figure 2
Figure 2
Flow cytometry detection of Annexin V /PI-stained cells after treatment with different concentrations of 5-FU for 72 hours. The sum of events in the upper right quadrant (late apoptotic/necrotic cells) and in the low right quadrant (early apoptotic cells) divided by the total number of events acquired. Panels A to I represent the results induced by different doses of 5-FU from 0, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50 to100 μg/mL separately.
Figure 3
Figure 3
Typical ATR-FTIR spectral signatures of MCF-7 cells treated with 5-fluorouracil.
Figure 4
Figure 4
ATR-FTIR spectra of MCF-7 cells treated with different concentrations of 5 -fluorouracil in Figure 4 T. From bottom to top correspond to drug concentrations including 0, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50 and 100 μg/mL. The increase in the band at 1741 cm−1 for cells exposed to increasing concentrations of 5-FU shows in a1 and the region at 1153–1170 cm−1 shows in b1. Corresponding secondary derivatives of the spectra are separately presented in a2 and b2 on the right.
Figure 5
Figure 5
Statistical correlation between the C = O/Amide I and apoptosis index (analysis performed from three independent experiments).
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References

    1. Avelar-Freitas BA, Almeida VG, Pinto MC, Mourao FA, Massensini AR, Martins-Filho OA, et al. Trypan blue exclusion assay by flow cytometry. Braz J Med Biol Res. 2014;47:307–15. doi: 10.1590/1414-431X20143437. - DOI - PMC - PubMed
    1. van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Methods Mol Biol. 2011;731:237–45. doi: 10.1007/978-1-61779-080-5_20. - DOI - PubMed
    1. Kerr WG. Analysis of apoptosis in hematopoietic stem cells by flow cytometry. Methods Mol Biol. 2008;430:87–99. doi: 10.1007/978-1-59745-182-6_6. - "VSports注册入口" DOI - PubMed
    1. Suggitt M, Bibby MC. 50 years of preclinical anticancer drug screening: empirical to target-driven approaches. Clin Cancer Res. 2005;11:971–81. - PubMed
    1. Mayr LM, Bojanic D. Novel trends in high-throughput screening. Curr Opin Pharmacol. 2009;9:580–8. doi: 10.1016/j.coph.2009.08.004. - DOI - PubMed

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