Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in VSports app下载. gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely V体育官网. .

. 2013 Nov;57(11):1908-17.
doi: 10.1002/mnfr.201300040. Epub 2013 Jun 19.

Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention

Soyoung Lim  1 , Jianteng Xu, Jaeyong Kim, Tzu-Yu Chen, Xiaoyu Su, Joseph Standard, Edward Carey, Jason Griffin, Betty Herndon, Benjamin Katz, John Tomich, Weiqun Wang
Affiliations

Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention

Soyoung Lim et al. Mol Nutr Food Res. 2013 Nov.

V体育官网入口 - Abstract

Scope: Anthocyanins, the natural pigments in plant foods, have been associated with cancer prevention. However, the content of anthocyanins in staple foods is typically low and the mechanisms by which they exert anticancer activity is not yet fully defined VSports手机版. .

Methods and results: We selected an anthocyanin-enriched purple-fleshed sweet potato clone, P40, and investigated its potential anticancer effect in both in vitro cell culture and in vivo animal model. In addition to a high level of total phenolics and antioxidant capacity, P40 possesses a high content of anthocyanins at 7. 5 mg/g dry matter. Treatment of human colonic SW480 cancer cells with P40 anthocyanin extracts at 0-40 μM of peonidin-3-glucoside equivalent resulted in a dose-dependent decrease in cell number due to cytostatic arrest of cell cycle at G1 phase but not cytotoxicity. Furthermore, dietary P40 at 10-30% significantly suppressed azoxymethane-induced formation of aberrant crypt foci in the colons of CF-1 mice in conjunction with, at least in part, a lesser proliferative PCNA and a greater apoptotic caspase-3 expression in the colon mucosal epithelial cells V体育安卓版. .

Conclusion: These observations, coupled with both in vitro and in vivo studies reported here, suggest anthocyanin-enriched sweet potato P40 may protect against colorectal cancer by inducing cell-cycle arrest, antiproliferative, and apoptotic mechanisms V体育ios版. .

Keywords: Anthocyanins; Colorectal cancer prevention; Mice; Purple-fleshed sweet potato; SW480 colon cancer cells. VSports最新版本.

PubMed Disclaimer

Conflict of interest statement

The authors have declared no conflict of interest.

Figures

Figure 1
Figure 1
Purple-fleshed sweet potato P40 versus cream-fleshed O’Henry and white-fleshed purple skin NC Japanese sweet potatoes grown at the John C. Pair Horticulture Research Center, Wichita, Kansas.
Figure 2
Figure 2
Representative HPLC chromatograms and MS data of anthocyanins detected in each of three sweet potato varities. Peonidin 3-glucoside was used as an internal standard (peak #5). Each anthocyanin peak is identified by ESI/MS/MS. The primary molecular weight, anthocyanin names, and m/z of the secondary fragment confirmation of the corresponded anthocyanin aglycones are listed in the inserted table.
Figure 3
Figure 3
Effects of P40 anthocyanin extract on cell growth and cell cycle progression in human colon SW480 cancer cells. (A) Treatment of cells with P40 anthocyanin extract at 10–40 μM of peonidin-3-glucoside equivalence for 48 hrs (■) versus the positive peonidin 3-glucoside control (□). Data are means ± SD, n = 3, Means within a treatment without a common letter are significantly different, p < 0.05. (B) Cells co-cultured with P40 anthocyanin extract at 10–40 μM of peonidin-3-glucoside equivalence for 48 hrs were analyzed by flow cytometry as described in the Materials and Methods. Data are means ± SD, n = 3, ** p<0.01 versus the vehicle control.
Figure 4
Figure 4
Effects of dietary P40 on the expression of PCNA and caspase-3 in the colon mucosal epithelial cells of mice injected with AOM. The top panel shows the representative immunohistochemical PCNA staining on colon tissue of CF-1 mice fed the basal AIN-93M diet (A), 30% of P40 diet (B), or caspase-3 staining on colon tissue of CF-1 mice fed the basal AIN-93M diet (D), 20% of P40 diet (E). The bottom panel is a quantification of the stained colon mucosal epithelial cells for PCNA (C) and caspase-3 (F), respectively. Data are means ± SD, n = 5, * p<0.05 or ** p<0.01 versus the basal AIM-93M diet-fed mice with AOM injection.
See this image and copyright information in PMC

References

    1. Bovell-Benjamin AC. Sweet potato: a review of its past, present, and future role in human nutrition. Adv Food Nutr Res. 2007;52:1–59. - PubMed
    1. Low JW, Arimond M, Osman N, Cunguara B, et al. A food-based approach introducing white-fleshed purple skin sweet potatoes increased vitamin A intake and serum retinol concentrations in young children in rural Mozambique. J Nutr. 2007;137:1320–1327. - "VSports app下载" PubMed
    1. Hotz C, Loechl C, Lubowa A, Tumwine JK, et al. Introduction of beta-carotene-rich orange sweet potato in rural Uganda resulted in increased vitamin A intakes among children and women and improved vitamin A status among children. J Nutr. 2012;142:1871–1880. - PubMed (V体育ios版)
    1. Mazza G, Miniati E. Anthocyanins in fruits, vegetables, and grains. CRC press; Boca Raton: 1993.
    1. Steed LE, Truong VD. Anthocyanin content, antioxidant activity, and selected physical properties of flowable purple-fleshed sweet potato purees. J Food Sci. 2008;73:S215–S221. - V体育安卓版 - PubMed

"VSports最新版本" Publication types

V体育ios版 - MeSH terms