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. 2012 Aug;45(4):386-96.
doi: 10.1111/j.1365-2184.2012.00826.x. Epub 2012 Jun 1.

VSports在线直播 - Equisetum arvense hydromethanolic extracts in bone tissue regeneration: in vitro osteoblastic modulation and antibacterial activity

C Bessa Pereira  1 , P S Gomes, J Costa-Rodrigues, R Almeida Palmas, L Vieira, M P Ferraz, M A Lopes, M H Fernandes
Affiliations

Equisetum arvense hydromethanolic extracts in bone tissue regeneration: in vitro osteoblastic modulation and antibacterial activity

"V体育平台登录" C Bessa Pereira et al. Cell Prolif. 2012 Aug.

Abstract

Objectives: Equisetum arvense preparations have long been used to promote bone healing. The aim of this work was to evaluate osteogenic and antibacterial effects of E VSports手机版. arvense hydromethanolic extracts. .

Materials and methods: Dried aerial components of E V体育安卓版. arvense were extracted using a mixture of methanol:water (1:1), for 26 days, yielding three extracts that were tested (10-1000 μg/ml) in human osteoblastic cells: E1, E2 and EM (a mixture of E1 and E2, 1:1). Cell cultures, performed on cell culture plates or over hydroxyapatite (HA) substrates, were assessed for osteoblastic markers. In addition, effects of the extracts on Staphylococcus aureus were addressed. .

Results: Solution E1 caused increased viability/proliferation and ALP activity at 50-500 μg/ml, and deleterious effects at levels ≥1000 μg/ml. E2 inhibited cell proliferation at levels ≥500 μg/ml V体育ios版. EM presented a profile between those observed with E1 and E2. In addition, E1, E2 and EM, 10-1000 μg/ml, inhibited expansion of S. aureus. Furthermore, E1, tested in HA substrates colonized with osteoblastic cells, causing increase in cell population growth (10-100 μg/ml). E1 also exhibited antibacterial activity against S. aureus cultured over HA. .

Conclusions: Results showed that E. arvense extracts elicited inductive effects on human osteoblasts while inhibiting activity of S VSports最新版本. aureus, suggesting a potentially interesting profile regarding bone regeneration strategies. .

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"V体育2025版" Figures

Figure 1
Figure 1
Cell viability/proliferation and ALP activity of hBMC cultures. Effect of E quisetum arvense extracts on viability/proliferation (a–c) and ALP activity (d) of hBMC cultured for 21 days in standard tissue culture plates. *Significantly different from control.
Figure 2
Figure 2
CLSM visualization of hBMC cultures. Cell morphology and proliferation of hBMC cultures treated with extract E1, over culture time. CLSM of cultures stained for F‐actin (green) and nuclei (red).
Figure 3
Figure 3
Presence of mineralized deposits in hBMC cultures treated with extract E1. (a) Representative images of cell layers following von Kossa staining (phosphate‐containing deposits); white bars, 100 μm. (b) SEM images after 21 days culture, showing cell layer‐associated mineral deposits; white bars, 15 μm. *Representative X‐ray spectrum of mineralized deposits, displaying Ca and P peaks.
Figure 4
Figure 4
Effects of Si on the behaviour of hBMC cultures. Effect of Si on viability/proliferation (a) and ALP activity (b) of hBMC cultures. *Significantly different from control.
Figure 5
Figure 5
X‐ray diffraction pattern of hydroxyapatite (a) SEM appearance of surface of HA‐dense discs used in the cell culture experiments (b) inset: macroscopic appearance, bar = 5 mm). HA colonized with hBMC grown in the presence of 50 μg/ml extract E1, at days 7 (c) 14 (d) and 21 (e) observed by CLSM.
Figure 6
Figure 6
Effect of Equisetum arvense E1 extract on behaviour of hBMC cultured on hydroxyapatite discs. (a) viability/proliferation; (b) ALP activity; (c) RT‐PCR analysis for expression of COL1, ALP, BMP‐2, RANKL, M‐CSF and OPG. *Significantly different from control.
Figure 7
Figure 7
Effect of Equisetum arvense extracts on proliferation of Staphylococcus aureus, after 24 h. (a) Antibacterial activity of E1, E2 and EM on cultures performed in standard tissue culture plates. (b) Antibacterial activity of extract E1 in cultures performed on hydroxyapatite discs. *Significantly different from control.
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