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. 2012;13(1):866-878.
doi: 10.3390/ijms13010866. Epub 2012 Jan 16.

Icariin ameliorates streptozotocin-induced diabetic retinopathy in vitro and in vivo

Hua Xin  1 , Feng Zhou  2 , Tao Liu  2 , Guang-Yong Li  2 , Jing Liu  2 , Zhe-Zhu Gao  2 , Guang-Yi Bai  2 , Hong Lu  1 , Zhong-Cheng Xin  2
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Icariin ameliorates streptozotocin-induced diabetic retinopathy in vitro and in vivo

Hua Xin et al. Int J Mol Sci. 2012.

V体育官网 - Abstract

This study investigated the effect of Icariin (ICA) supplementation on diabetic retinopathy (DR) in a streptozotocin-induced diabetic rat model system. Fifty Sprague Dawley rats were randomly distributed into a control group and a streptozotocin-induced diabetes group. Diabetic rats were randomly divided into two groups; one group received ICA 5 mg/kg/day for 12 weeks by oral gavage; the other group received saline gavage as a placebo VSports手机版. Retinal morphological changes, endothelial markers (RECA), collagen IV (Col-IV), vascular endothelial growth factor (VEGF), and neuropathic changes (Thy-1 and Brn3a expression) of the retinal ganglion cells (RGCs) were investigated. The effects of ICA at various concentrations (0, 10(1), 10(2), 10(3) nmol/mL) on neurite growth were investigated also in retinal ganglion cells (RGC) cultured from both diabetic and normal animals. Numerous pathological changes (deceased expression of RECA, VEGF, Thy-1, and Brn3a as well as decreased Collagen IV and Müller cell content) were noted in the retinal vessels of diabetic rats; these changes were attenuated in diabetic animals that received ICA. ICA enhanced neurite growth in RGC from both normal rats and diabetic rats in a dose dependent fashion. ICA may be useful in the treatment of diabetic retinopathy. Further investigations are indicated. .

Keywords: diabetes; diabetic retinopathy; icariin; retina; streptozotocin. V体育安卓版.

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V体育官网 - Figures

Figure 1
Figure 1
The effect of icariin on morphological changes of disbetic retinopathy. The notable morphological changes were observed in diabetic retina, however, these changes were improved by ICA treated diabetic retina and the average thickness of retina in different groups.
Figure 2
Figure 2
The effects of ICA on Col IV, RECA and VEFG expression micro blood vessels in diabetic retina. RECA, Col-IV, and VEGF were used to check the retinal microvessls. RECA and Col-IV clearly demonstrated the distribution of retinal vessels. As described in the text, DM decreased the blood vessels density in retina while ICA improved these conditions. It is noted that ICA improved the expression of VEGF significantly.
Figure 3
Figure 3
The effects of ICA on RGCs Thy-1 and Brna-3 and CA II expression in Diabetic retina. Thy-1 and Brn3a were used to detect the RGCs (in up panel and lower panel), CA-II was used to detect the Müller cells (in middle panel). Diabetes significantly decreased the RGCs and increased Müller cells in the retina, while ICA improved both pathological situations.
Figure 4
Figure 4
The neurites sprout from retina after stimulation with ICA in normal rats and diabetic rats. Paired comparisons were made between retina derived from normal control and DM rats at the treatment of 0, 10, 100 and 1000 nmol/mL ICA, all retina treated with ICA had significantly longer average neurite length in different dose of ICA in normal rats and diabetic rats when compared to DM group.
Scheme 1
Scheme 1
The chemical structures of Icariin.
See this image and copyright information in PMC

References

    1. Congdon N., O’Colmain B., Klaver C.C., Klein R., Muñoz B., Friedman D.S., Kempen J., Taylor H.R., Mitchell P. Eye Diseases Prevalence Research Group. Causes and prevalence of visual impairment among adults in the United States. Arch. Ophthalmol. 2004;122:477–485. - PubMed
    1. Marshall S.M., Flyvbjerg A. Prevention and early detection of vascular complications of diabetes. Br. Med. J. 2006;333:475–480. - PMC - PubMed
    1. Klein R., Klein B.E., Moss S.E. Epidemiology of proliferative diabetic retinopathy. Diabetes Care. 1992;15:1875–1891. - V体育平台登录 - PubMed
    1. Frank R.N. Diabetic retinopathy. N. Engl. J. Med. 2004;350:48–58. - PubMed
    1. Curtis T.M., Gardiner T.A., Stitt A.W. Microvascular lesions of diabetic retinopathy: Clues towards understanding pathogenesis? Eye (Lond.) 2009;23:1496–1508. - V体育平台登录 - PubMed

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