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. 2011:2011:521852.
doi: 10.1155/2011/521852. Epub 2011 Oct 20.

A Human Head and Neck Squamous Cell Carcinoma Cell Line with Acquired cis-Diamminedichloroplatinum-Resistance Shows Remarkable Upregulation of BRCA1 and Hypersensitivity to Taxane

Yuriko Saiki  1 , Takenori Ogawa, Kiyoto Shiga, Makoto Sunamura, Toshimitsu Kobayashi, Akira Horii
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A Human Head and Neck Squamous Cell Carcinoma Cell Line with Acquired cis-Diamminedichloroplatinum-Resistance Shows Remarkable Upregulation of BRCA1 and Hypersensitivity to Taxane

Yuriko Saiki et al. Int J Otolaryngol. 2011.

V体育ios版 - Abstract

Recently, an inverse relationship between resistance to platinum-based chemotherapeutic agents and taxanes has been implicated in breast and ovarian cancers, and a possible pivotal role for BRCA1 has also been suggested. Because cis-diamminedichloroplatinum (CDDP) and taxanes are the most active antitumor agents against head and neck squamous cell carcinoma (HNSCC), we analyzed the sensitivity of nine HNSCC cell lines and their previously established derived CDDP-resistant cell lines to two representative taxanes: docetaxel and paclitaxel. None of the nine original cell lines showed any cross resistance between CDDP and taxanes, but one of the CDDP-resistant cell lines, RPMI2650CR, demonstrated hypersensitivity to both taxanes when compared to the parental cell line, RPMI2650 VSports手机版. Furthermore, RPMI2650CR exhibited increased expression of BRCA1. These data suggest that (i) taxanes are a good candidate for a second-line therapeutic drug for HNSCC patients with acquired CDDP resistance and (ii) BRCA1 can be a candidate marker for predicting an inverse CDDP/taxane sensitivity phenotype in HNSCC. .

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Figures

Figure 1
Figure 1
Cell viability with chemotherapeutic agents was assessed by MTT assay. RPMI2650CR shows higher resistance to cisplatin that the parental RPMI2650 (a) but has higher sensitivities to both docetaxel (b) and paclitaxel (c).
Figure 2
Figure 2
Western blot analysis of BRCA1 in HNSCC cell lines and their derived CDDP-resistant (CR) cell lines. Marked upregulation of BRCA1 (220 kDa) was observed only in the RPMI2650CR cell line. Beta actin (ACTB) was used as an internal control.
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References

    1. Forastiere AA. Chemotherapy in the treatment of locally advanced head and neck cancer. Journal of Surgical Oncology. 2008;97(8):701–707. - PubMed
    1. Kelland L. The resurgence of platinum-based cancer chemotherapy. Nature Reviews Cancer. 2007;7(8):573–584. - PubMed
    1. Ogawa T, Ogawa K, Shiga K, et al. Upregulation of IGF2 is associated with an acquired resistance for cis-diamminedichloroplatinum in human head and neck squamous cell carcinoma. European Archives of Oto-Rhino-Laryngology. 2010;267(10):1599–1606. - PubMed
    1. Stordal B, Pavlakis N, Davey R. A systematic review of platinum and taxane resistance from bench to clinic: an inverse relationship. Cancer Treatment Reviews. 2007;33(8):688–703. - VSports - PubMed
    1. Posner MR, Hershock DM, Blajman CR, et al. Cisplatin and fluorouracil alone or with docetaxel in head and neck cancer. New England Journal of Medicine. 2007;357(17):1705–1715. - PubMed

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