Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official VSports app下载. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely V体育官网. .

. 2009 Feb 15;69(4):1302-13.
doi: 10.1158/0008-5472.CAN-08-2741. Epub 2009 Feb 3.

Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature (VSports在线直播)

Emmanuelle Charafe-Jauffret  1 , Christophe Ginestier, Flora Iovino, Julien Wicinski, Nathalie Cervera, Pascal Finetti, Min-Hee Hur, Mark E Diebel, Florence Monville, Julie Dutcher, Marty Brown, Patrice Viens, Luc Xerri, François Bertucci, Giorgio Stassi, Gabriela Dontu, Daniel Birnbaum, Max S Wicha
Affiliations

VSports注册入口 - Breast cancer cell lines contain functional cancer stem cells with metastatic capacity and a distinct molecular signature

Emmanuelle Charafe-Jauffret et al. Cancer Res. .

Abstract

Tumors may be initiated and maintained by a cellular subcomponent that displays stem cell properties. We have used the expression of aldehyde dehydrogenase as assessed by the ALDEFLUOR assay to isolate and characterize cancer stem cell (CSC) populations in 33 cell lines derived from normal and malignant mammary tissue VSports手机版. Twenty-three of the 33 cell lines contained an ALDEFLUOR-positive population that displayed stem cell properties in vitro and in NOD/SCID xenografts. Gene expression profiling identified a 413-gene CSC profile that included genes known to play a role in stem cell function, as well as genes such as CXCR1/IL-8RA not previously known to play such a role. Recombinant interleukin-8 (IL-8) increased mammosphere formation and the ALDEFLUOR-positive population in breast cancer cell lines. Finally, we show that ALDEFLUOR-positive cells are responsible for mediating metastasis. These studies confirm the hierarchical organization of immortalized cell lines, establish techniques that can facilitate the characterization of regulatory pathways of CSCs, and identify potential stem cell markers and therapeutic targets. .

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. Global gene expression profiling of 33 breast cell lines analyzed by the ALDEFLUOR assay
Hierarchical clustering of 33 breast cell lines and 13,550 genes/ESTs based on mRNA expression levels. A. The dendrogram of samples represents overall similarities in gene expression profiles. Two large groups of samples are evidenced by clustering. Name of cell lines is colored as follows: blue for luminal (n=12), red for basal (n=10), brown for mesenchymal (n=6) cell lines according to the correlation of expression profile of each cell line with each Ross and Perou centroid (i.e. molecular subtype). Five cell lines were not attributed any subtype (name in grey). The ER, ERBB2, and ALDEFLUOR status of breast cell lines are represented according to a color ladder (for ER and ERBB2 status: negative, white; positive, black; unavailable, oblique feature; for ALDEFLUOR status: a color scale shown at the bottom of the dendrogram relates the percentage of ALDELFUOR-positive cells found in each breast cell line). B. Comparison of the ALDEFLUOR status with the molecular subtypes of breast cell lines revealed a strong correlation between the basal/mesenchymal subtypes and the presence of ALDEFLUOR-positive cells (p-value=0.0006).
Fig. 2
Fig. 2. The ALDEFLUOR-positive cell populations from breast cancer cell lines (MDA-MB-453, SUM159) have cancer stem cell properties
A-B. Tumor growth curves were plotted for different numbers of cells injected (for MDA-MB-453: 50,000 cells, 5,000 cells, and 500 cells and for SUM159: 100,000 cells, 10,000 cells, and 1,000 cells) and for each population (ALDEFLUOR-positive, ALDEFLUOR-negative, unseparated). Tumor growth kinetics correlated with the latency and size of tumor formation and the number of ALDEFLUOR-positive cells. C-D. H&E staining of ALDEFLUOR-positive cells' injection site, revealing presence of tumor cells (Ca: MDA-MB-453 ALDEFLUOR-positive cells' injection site, and Da: SUM59 ALDEFLUOR-positive cells' injection site). Cb, Db. The ALDEFLUOR-negative cells' injection site contained only residual Matrigel, apoptotic cells, and mouse tissue (Cb: MDA-MB-453 ALDEFLUOR-negative cells' injection site, and Db: SUM59 ALDEFLUOR-negative cells' injection site). Data represent mean ± SD.
Fig. 3
Fig. 3. Validation of gene expression results by quantitative RT-PCR
A-B. To confirm our gene expression results, we measured in a set of five breast cancer cell lines sorted for the ALDEFLUOR phenotype the expression of five discriminator genes overexpressed in ALDEFLUOR-positive populations (CXCR1/IL8RA, FBXO21, NFYA, NOTCH2 and RAD51L1) by quantitative RT-PCR. The quantitative RT-PCR expression levels of CXCR1 and FBXO21 are presented in this figure and the ones of NFYA, NOTCH2, and RAD51L1 are presented in Supplementary Fig. 8. Gene expression levels measured by quantitative RT-PCR confirm the results obtained using DNA microarrays with an increase of CXCR1 and FBXO21 mRNA level in the ALDEFLUOR-positive population compared to the ALDEFLUOR-negative population (p<0.05).
Fig. 4
Fig. 4. Role of the IL8/CXCR1 axis in the regulation of breast cancer stem cells
A. Effect of IL8 treatment on tumorosphere formation of three different cell lines (HCC1954, SUM159, MDA-MB-453). IL8 treatment increased the formation of primary and secondary tumorospheres in a dose-dependent manner. B. Effect of IL8 treatment on the ALDEFLUOR-positive population of four different cell lines cultured in adherent conditions. IL8 increased the ALDEFLUOR-positive population in a dose-dependent manner in each of the four cell lines analyzed (* p<0.05/ ** p<0.01, statistically significant differences from the control group).
Fig. 5
Fig. 5. ALDEFLUOR-positive cells display increased metastatic potential
A. The IL8/CXCR1 axis is involved in cancer stem cell invasion. The role of the IL8/CXCR1 axis in invasion was assessed by a Matrigel invasion assay using serum or IL8 as attractant for three different cell lines (HCC1954, MDA-MB-453, SUM159). ALDEFLUOR-positive cells were 6- to 20-fold more invasive than ALDEFLUOR-negative cells (p<0.01). When using IL8 (100 ng/ml) as attractant, we observed a significant increase of ALDEFLUOR-positive cells invading through Matrigel compared to serum as attractant (p<0.05). In contrast IL8 had no effect on the invasive capacity of the ALDEFLUOR-negative population. B-C. The ALDEFLUOR-positive population displayed increased metastatic potential. Ba-c. Quantification of the normalized photon flux measured at weekly intervals following inoculation of 100,000 luciferase infected cells from each group (ALDEFLUOR-positive, ALDEFLUOR-negative, unseparated). Mice inoculated with ALDEFLUOR-positive cells developed several metastasis localized at different sites (bone, muscle, lung, soft tissue) and displayed a higher photon flux emission than mice inoculated with unseparated cells, which developed no more than one metastasis per mouse. In contrast, mice inoculated with ALDEFLUOR-negative cells developed only an occasional small metastasis, which was limited to lymph nodes. C. Histologic confirmation, by H&E staining, of metastasis in bone (Ca), soft tissue (Cb) and muscle (Cc) resulting from injection of ALDEFLUOR-positive cells.
See this image and copyright information in PMC

"VSports" References

    1. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell. 2000;100:57–70. - PubMed
    1. Neve RM, Chin K, Fridlyand J, et al. A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer Cell. 2006;10:515–27. - PMC - PubMed
    1. Bonnet D, Dick JE. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med. 1997;3:730–7. - "V体育安卓版" PubMed
    1. Glinsky GV. Stem cell origin of death-from-cancer phenotypes of human prostate and breast cancers. Stem Cell Rev. 2007;3:79–93. - PubMed (V体育官网)
    1. Jaiswal S, Traver D, Miyamoto T, Akashi K, Lagasse E, Weissman IL. Expression of BCR/ABL and BCL-2 in myeloid progenitors leads to myeloid leukemias. Proc Natl Acad Sci USA. 2003;100:10002–7. - V体育官网 - PMC - PubMed

Publication types

MeSH terms