Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR

Y-N Wang; L Yu; X-S Zhao; W Zhang; J Wan; B Yu

Issue

Vol. 61 No. 3: Issue 3

Issue Published : May 18, 2016

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Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR (V体育官网入口)

Y-N Wang

Shenzhen Peking University Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute Shenzhen China

L Yu

Peking University Shenzhen Hospital Department of gynaecology and obstetrics Shenzhen

X-S Zhao

Peking University Peking University People's Hospital, Peking University Institute of Hematology, Collaborative Innovation Center of Hematology Beijing China

W Zhang

Shenzhen Peking University Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute Shenzhen China

J Wan

Shenzhen Peking University Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute Shenzhen China

B Yu

Peking University Shenzhen Hospital Department of Dermatology Shenzhen China

Corresponding Author(s) : Y-N Wang

Cellular and Molecular Biology, Vol. 61 No VSports. 3: Issue 3 Article Published : June 24, 2015 .

"VSports注册入口" Abstract

copies in the PCR reaction. Our study provided a standardized quantification method for plasma miRNAs which might be used as biomarker in many diseases research.

Keywords

C. elegans microRNA (Cel-miR) MicroRNA-153 (miR-153) MicroRNA-486 (miR-486) Plasma microRNA Quantitative reverse transcription-PCR (qRT-PCR) Taqman probe.

Wang, Y.-N., Yu, L., Zhao, X.-S., Zhang, W., Wan, J., & Yu, B. (2015). Establishment of plasma microRNA detection method by using taqman probe based quantitative reverse transcription PCR. Cellular and Molecular Biology, 61(3), 51–56. Retrieved from https://www.cellmolbiol.org/index.php/CMB/article/view/669