Leia A Jones; Gillian E Conway; Aimy  Nguyen-Chi; Stephanie  Burnell; Gareth J Jenkins; R. Steven  Conlan; Shareen H Doak

doi:10.14715/cmb/2023.69.4.28

Issue

Vol. 69 No. 4: Issue 4

Issue Published : June 17, 2023

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Investigating STEAP2 as a potential therapeutic target for the treatment of aggressive prostate cancer.

https://doi.org/10.14715/cmb/2023.69.4.28

Leia A Jones

Institute of Life Science, Swansea University Medical School, Faculty of Medicine, Health and Life Sciences, Singleton Park, Swansea, SA2 8PP, Wales, UK

Gillian E Conway

Institute of Life Science, Swansea University Medical School, Faculty of Medicine, Health and Life Sciences, Singleton Park, Swansea, SA2 8PP, Wales, UK

Corresponding Author(s) : Shareen H Doak

s.h.doak@www.qiuluzeuv.cn Cellular and Molecular Biology, Vol V体育官网. 69 No. 4: Issue 4 Article Published : April 30, 2023 .

Abstract

The expression of six transmembrane epithelial antigen of the prostate (STEAP2) is increased in prostate cancer when compared to normal tissue, suggesting a role for STEAP2 in disease progression. This study aimed to determine whether targeting STEAP2 with an anti-STEAP2 polyclonal antibody (pAb) or CRISPR/Cas9 knockout influenced aggressive prostate cancer traits. Gene expression analysis of the STEAP gene family was performed in a panel of prostate cancer cell lines; C4-2B, DU145, LNCaP and PC3. The highest increases in STEAP2 gene expression were observed in C4-2B and LNCaP cells (p<0. 001 and p<0. 0001 respectively) when compared to normal prostate epithelial PNT2 cells. These cell lines were treated with an anti-STEAP2 pAb and their viability assessed. CRISPR/Cas9 technology was used to knockout STEAP2 from C4-2B and LNCaP cells and viability, proliferation, migration and invasion assessed. When exposed to an anti-STEAP2 pAb, cell viability significantly decreased (p<0. 05). When STEAP2 was knocked out, cell viability and proliferation was significantly decreased when compared to wild-type cells (p<0. 001) VSports手机版. The migratory and invasive potential of knockout cells were also decreased. These data suggest that STEAP2 has a functional role in driving aggressive prostate cancer traits and could provide a novel therapeutic target for the treatment of prostate cancer.

VSports app下载 - Keywords

Prostate cancer STEAP2 biomarker CRISPR/Cas9 antibody proliferation metastasis invasion

Jones, L. A., Conway, G. E., Nguyen-Chi, A. ., Burnell, S. ., Jenkins, G. J., Conlan, R. S. ., & Doak, S. H. (2023). Investigating STEAP2 as a potential therapeutic target for the treatment of aggressive prostate cancer. Cellular and Molecular Biology, 69(4), 179–187. https://doi.org/10.14715/cmb/2023.69.4.28