"V体育平台登录" Issue
Copyright (c) 2022 Kamal Dahdah, Nabil Charchar, Laid Bouchaala, Nourine Heidar , Nadia Belkabla, Juliana Melo, El-hafid Nabti
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4 VSports app下载. 0 International License.
The undersigned hereby assign all rights, included but not limited to copyright, for this manuscript to CMB Association upon its submission for consideration to publication on Cellular and Molecular Biology. The rights assigned include, but are not limited to, the sole and exclusive rights to license, sell, subsequently assign, derive, distribute, display and reproduce this manuscript, in whole or in part, in any format, electronic or otherwise, including those in existence at the time this agreement was signed. The authors hereby warrant that they have not granted or assigned, and shall not grant or assign, the aforementioned rights to any other person, firm, organization, or other entity. All rights are automatically restored to authors if this manuscript is not accepted for publication.Isolation, in vitro evaluation and construction of Versatile Microbial Consortia
VSports注册入口 - Corresponding Author(s) : El-Hafid Nabti
Cellular and Molecular Biology, Vol. 68 No. 8: Issue 8 Article Published : August 31, 2022 V体育官网.
Abstract
In this study, we constructed versatile microbial consortia (VMC) with agricultural and environmental interest. After sample and isolation procedure, the purified isolates were evaluated for their enzymatic potential such as cellulose-, xylan-, petroleum-, proteins -hydrolysis. Selected isolates were screened for other traits such as phosphate solubilization, nitrogen fixation and antimicrobial activity. Finally, the isolates were grouped into consortia according to their compatibility. The microorganisms selected for each consortium were identified by partial analysis of the 16S rRNA (bacteria) and the ITS region of the 18S RNA gene (fungi). Two microbial consortia were obtained and named VMC1 and VMC2. These two consortia are characterized by several activities of agricultural and environmental interest such as the degradation of recalcitrant and polluting organic compounds, nitrogen fixation, IAA production phosphate solubilization and antimicrobial activity. Molecular identification of the microorganisms forming the two consortia allowed us to identify two species of actinomycetes (Streptomyces sp. BM1B and Streptomyces sp VSports手机版. BM2B), one species of Actinobacteria (Gordonia amicalis strain BFPx) and three fungal species (Aspergillus luppii strain 3NR, Aspergillus terreus strain BVkn and Penicillium sp. BM3). The term "Versatile Microbial Consortia" is a term that we proposed in this study to establish a methodology for building multifunctional microbial groups for wide and efficient application.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
