VSports最新版本 - Analysis of Fusion Genes by NanoString System: A Role in Lung Cytology?

Next Generation Sequencing for Gene Fusion Analysis in Lung Cancer: A Literature Review

Bruno1, Fontanini2 2020DiagnosticsSelf Cite13611310Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…Treatment decisions can be made when ALK IHC results are clearly positive, whereas positive ROS1 and NTRK IHC results have to be confirmed by orthogonal techniques [ 2 , 3 ]. Although multiplexing has been improved, FISH and IHC usually require a test for each gene to analyze, expert pathologists are needed and results can be influenced by interobserver variation [ 16 ]. RT-PCR allows a highly sensitive detection of fusion transcripts at RNA levels, but it requires primer pairs specific for the known fusion to investigate. …”Section: Introductionmentioningconfidence: 99%“…In this context, it is possible to discriminate NGS and non-NGS methods. Among non-NGS methods the NanoString nCounter System is one of the most valuable for gene fusion analysis [ 13 , 16 ]. It is based on a digital color-coded barcode technology allowing to directly count specific mRNA molecules without any retro-transcription or amplification step, thus being adequate also for the analysis of degraded clinical samples [ 18 ]. …”Section: Introductionmentioningconfidence: 99%

Next Generation Sequencing for Gene Fusion Analysis in Lung Cancer: A Literature Review

Bruno1, Fontanini2 2020DiagnosticsSelf Cite13611310Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstract“…In this field, the NanoString system reviewed by Ali et al 1 is very interesting and promising compared with the classic gold standard fluorescent in situ hybridization (FISH) tests. Ali et al 1 argued that FISH is expensive, requires technical expertise that may not be readily available in a histopathology laboratory, and is not amenable to multiplexed analysis. 1 We intend to provide an alternative point of view about these drawbacks of FISH testing in NSCLC samples. …”mentioningconfidence: 99%“…FISH also allows the interpretation of samples with few tumor cells, including cytology samples that can be recognized among nontumor cells by trained pathologists, whereas the detection of low percentages of rearranged alleles could be challenging using methods requiring DNA/ RNA extraction. 3 According to Ali et al, 1 FISH is not amenable to multiplexed analysis. Nevertheless, besides classical breakapart probes designed to analyze a single gene, multiplexed FISH probes are now developed to concurrently analyze several oncogenes as ALK and ROS1 genes to date, using different fluorescent probe combinations. …”mentioningconfidence: 99%“…Particularly, NanoString can provide an alternative molecular diagnostic approach that can help to control costs, eliminate unnecessary testing, and improve turnaround time. 1,6 Anyway, it always has to be considered that the adoption of a specific technique depends on local resources, expertise of the laboratory, number of cases per year, and local reimbursement policy. Greta Alì, MD 1 ; Rossella Bruno, PhD 2 ; Gabriella Fontanini, MD 2,3 Accepted for publication July 19, 2018. doi: 10. 5858/arpa. 2018-0357-LE…”mentioningconfidence: 99%See 1 more Smart Citation

VSports - NanoString System Versus Multiplex Fluorescent In Situ Hybridization for Gene Fusion Diagnosis in Lung Cancer Cytology Samples: Competing or Complementary Methods?

Lambros1, Uguen2 2018Archives of Pathology &Amp; Laboratory Medicine0000Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

Bird’s eye view of modern cytopathology: Report from the seventh international Molecular Cytopathology Meeting in Naples, Italy, 2018

Pisapia1, Bellevicine2, Malapelle3 et al. 2019Cancer Cytopathology4040Get access via publisherAdd to dashboardCite Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. Show abstract Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task. show abstractScite is an AI-powered research tool that helps researchers better discover and evaluate scientific literature through Smart Citations—a revolutionary system that shows whether articles support, contrast, or simply mention a given claim. Founded in 2018, and now part of Research Solutions, Scite has indexed over 1. 4 billion citations and partnered with more than 30 major publishers to provide researchers with unparalleled access to scientific literature VSports. With its Scite Assistant, Smart Citation Index, and advanced search capabilities, the platform addresses critical challenges such as information overload and research reproducibility. Trusted by two million active users worldwide, Scite is reshaping how researchers interact with scholarly content—building ethical, transparent AI tools that support rigorous, copyright-compliant research.