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. 1997 Aug 22;90(4):625-34.
doi: 10.1016/s0092-8674(00)80524-3.

ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation

K Kimura  1 , T Hirano
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ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation

K Kimura et al. Cell. .
Free article

V体育官网入口 - Abstract

13S condensin is a five-subunit protein complex that plays a central role in mitotic chromosome condensation in Xenopus egg extracts. Two core subunits of this complex, XCAP-C and XCAP-E, belong to an emerging family of putative ATPases, the SMC family. We report here that 13S condensin has a DNA-stimulated ATPase activity and exhibits a high affinity for structured DNAs such as cruciform DNA. 13S condensin is able to introduce positive supercoils into a closed circular DNA in the presence of bacterial or eukaryotic topoisomerase I VSports手机版. The supercoiling reaction is ATP-dependent. We propose that 13S condensin wraps DNA in a right-handed direction by utilizing the energy of ATP hydrolysis. This reaction may represent a key mechanism underlying the compaction of chromatin fibers during mitosis. .

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