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Comparative Study
. 1996 Jan 15;15(2):265-75.

The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution

K Colwill  1 , T Pawson, B Andrews, J Prasad, J L Manley, J C Bell, P I Duncan
Affiliations
Comparative Study

The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution

K Colwill et al. EMBO J. .

Abstract

Mammalian Clk/Sty is the prototype for a family of dual specificity kinases (termed LAMMER kinases) that have been conserved in evolution, but whose physiological substrates are unknown. In a yeast two-hybrid screen, the Clk/Sty kinase specifically interacted with RNA binding proteins, particularly members of the serine/arginine-rich (SR) family of splicing factors. Clk/Sty itself has an serine/arginine-rich non-catalytic N-terminal region which is important for its association with SR splicing factors. In vitro, Clk/Sty efficiently phosphorylated the SR family member ASF/SF2 on serine residues located within its serine/arginine-rich region (the RS domain). Tryptic phosphopeptide mapping demonstrated that the sites on ASF/SF2 phosphorylated in vitro overlap with those phosphorylated in vivo. Immunofluorescence studies showed that a catalytically inactive form of Clk/Sty co-localized with SR proteins in nuclear speckles. Overexpression of the active Clk/Sty kinase caused a redistribution of SR proteins within the nucleus. These results suggest that Clk/Sty kinase directly regulates the activity and compartmentalization of SR splicing factors. VSports手机版.

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References (VSports手机版)

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