日 <u dropzone="lb2uA"></u> Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 1995 Jul;15(7):3813-22.
doi: 10.1128/MCB.15.7.3813.

Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system (V体育平台登录)

S M Hollenberg  1 , R Sternglanz, P F Cheng, H Weintraub
Affiliations

Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system (VSports app下载)

S M Hollenberg et al. Mol Cell Biol. 1995 Jul.

Abstract

With modified two-hybrid technology, we have isolated a member of a new family of basic helix-loop-helix (bHLH) transcription factors. Thing1 (Th1) was identified in a screen of a mouse embryo cDNA library as a partner for the Drosophila E protein daughterless. RNA in situ hybridization and reverse transcriptase-PCR demonstrate a stage- and tissue-specific distribution for the expression of Th1. Although tissue specific, the expression pattern of Th1 is fairly complex. During development, Th1 mRNA is widely expressed in extraembryonic tissues, portions of the heart, autonomic ganglia, the gut, and pharyngeal arches. At embryonic day 7. 5 (E7. 5), extraembryonic derivatives show robust Th1 expression. By E8. 5, expression in the embryonic heart becomes detectable. During the next 2 days of development, the signal also includes gut and pharyngeal arches. Predominant expression at E13. 5 is in neural crest derivatives, especially the autonomic nervous system and adrenal medulla. Expression of Th1 persists in the adult, in which it is localized to the smooth muscle cells of the gut. In vitro, Th1 protein recognizes a set of DNA sites that are more degenerate than has been determined for other bHLH factors, indicating a reduced binding specificity. Transient transfection of NIH 3T3 cells with GAL4-Th1 fusions reveals a repression activity mediated by the Th1 bHLH domain VSports手机版. In combination, these properties define Th1 as a new bHLH protein with a unique set of properties. .

PubMed Disclaimer

"VSports在线直播" References

    1. Semin Cancer Biol. 1993 Dec;4(6):341-7 - VSports最新版本 - PubMed
    1. Mol Cell Biol. 1991 Aug;11(8):4104-10 - PubMed
    1. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7066-70 - PubMed
    1. Genes Dev. 1994 Apr 15;8(8):970-80 - PubMed
    1. Trends Genet. 1994 Aug;10(8):286-92 - V体育2025版 - PubMed

Publication types

MeSH terms (VSports手机版)

Associated data