Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site VSports app下载. .

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2021 Dec 16:2021:8069930.
doi: 10.1155/2021/8069930. eCollection 2021.

V体育2025版 - Icariin and Icariside II Reciprocally Stimulate Osteogenesis and Inhibit Adipogenesis of Multipotential Stromal Cells through ERK Signaling

Dawei Zhang  1   2 , Ning Zhao  1 , Chao Wan  1 , Jikun Du  1 , Jiantao Lin  2 , Hongbo Wang  1
Affiliations

V体育平台登录 - Icariin and Icariside II Reciprocally Stimulate Osteogenesis and Inhibit Adipogenesis of Multipotential Stromal Cells through ERK Signaling

Dawei Zhang et al. Evid Based Complement Alternat Med. .

Abstract (VSports注册入口)

Herba Epimedii is a famous Chinese herbal medicine for treating bone diseases VSports手机版. Icariin and icariside II, the main chemical constituents, have attracted great attention from scientists for their potential as antiosteoporosis agents. Our study aimed to evaluate their effects on the lineage commitment of multipotential stromal cells (MSCs). The osteogenesis and adipogenesis of MSCs were assessed by ALP activity, calcium deposition, and adipocyte formation. The expression profiles and levels of osteogenic and adipogenic specific genes were evaluated by cDNA microarray and quantitative real-time PCR. The involvement of extracellular signal-regulated kinase (ERK) signaling was studied by enzyme-linked immunosorbent assay. Icariin and icariside II significantly increased ALP activity and mineralization during osteogenic differentiation of MSCs. Runx2, Col1, and Bmp2 were upregulated in the presence of icariin and icariside II. Meanwhile, they downregulated Pparg, Adipsin, and Cebpb expression during adipogenic differentiation. cDNA microarray revealed 57 differentially expressed genes during lineage commitment of MSCs. In addition, icariin and icariside II enhanced the phosphorylation of ERK, and the above biological effects were blocked by ERK inhibitor U0126. Icariin and icariside II may drive the final lineage commitment of MSCs towards osteogenesis and inhibit adipogenesis through the ERK signaling pathway. Both of them exert multiple osteoprotective effects and deserve more attention for their medicinal and healthcare prospects. .

PubMed Disclaimer

"V体育官网入口" Conflict of interest statement

The authors declare that they have no conflicts of interest.

VSports注册入口 - Figures

Figure 1
Figure 1
Icariin (a) and icariside II (b) stimulated the proliferation of MSCs. NaF, 1 µM; ES, 10 nM 17β-estradiol. ∗P < 0.05 vs. control.
Figure 2
Figure 2
Icariin (a) and icariside II (b) increased ALP activity during osteogenesis of MSCs. NaF, 1 µM; ES, 10 nM 17β-estradiol. ∗P < 0.05 and ∗∗p < 0.01 vs. PBS control.
Figure 3
Figure 3
Quantification of alizarin red S staining during osteogenesis of MSCs. (a–d). Alizarin red S staining (100×): (a) Osteogenic supplements (OS) medium. (b) OS + NaF (1 µM). (c) OS + icariin (1 µM). (d) OS + icariside II (1 µM). (e-f) Quantification of ARS staining: (e) Icariin. (f) Icariside II. ∗P < 0.05 and ∗∗p < 0.01 vs. the OS group. Scale bar = 20 µM.
Figure 4
Figure 4
Adipocyte-like cell formation during adipogenesis of MSCs. (a–d) Oil red O staining (100×). (a) AS medium. (b) AS + ES (10 nM). (c) AS + icariin (1 µM). (d) AS + icariside II (1 µM). (e-f) Quantification of oil red O staining: (e) Icariin. (f) Icariside II. ∗P < 0.05 and ∗∗p < 0.01 vs. the AS group. Scale bar = 40 µM.
Figure 5
Figure 5
Representative gene expression during osteogenesis (a) and adipogenesis (b) of MSCs in the presence of icariin and icariside II. AS, adipogenic supplement; OS, osteogenic supplement; ES, 10 nM 17β-estradiol; N, growth medium; ICA, icariin; IS2, icariside II. #P < 0.01 vs. the N group. ∗P < 0.05 and ∗∗p < 0.01 vs. the AS/OS group.
Figure 6
Figure 6
ERK signaling was activated during osteogenesis and adipogenesis of MSCs in the presence of icariin and icariside II. (a) ALP activity. (b-c) Gene expression of Runx2 and Pparg. (d) Level of ERK phosphorylation by ELISA. ICA, icariin; IS2, icariside II; U0126, ERK1/2 inhibitor. #P < 0.01 vs. the control group. ∗P < 0.05 vs. the ICA/IS2 group.
Figure 7
Figure 7
PANTHER functional analysis during osteogenic differentiation of MSCs. (a) Biological process. (b) Molecular function.
Figure 8
Figure 8
PANTHER functional analysis during adipogenic differentiation of MSCs. (a) Biological process. (b) Molecular function.
See this image and copyright information in PMC

References

    1. Allen M. R., Burr D. B. Bone modeling and remodeling. In: Allen M. R., Burr D. B., editors. Basic and Applied Bone Biology . Cambridge, MA, USA: Academic Press; 2013. pp. 75–90. - DOI
    1. Arceo-Mendoza R. M., Camacho P. M. Postmenopausal osteoporosis. Endocrinology and Metabolism Clinics of North America . 2021;50(2):167–178. doi: 10.1016/j.ecl.2021.03.009. - DOI - PubMed
    1. Słupski W., Jawień P., Nowak B. Botanicals in postmenopausal osteoporosis. Nutrients . 2021;13(5):p. 1609. doi: 10.3390/nu13051609. - DOI - PMC - PubMed
    1. Wang L., Li Y., Guo Y., et al. Herba epimedii: an ancient Chinese herbal medicine in the prevention and treatment of osteoporosis. Current Pharmaceutical Design . 2016;22(3):328–349. doi: 10.2174/1381612822666151112145907. - DOI - PubMed
    1. Xie F., Wu C.-F., Lai W.-P., et al. The osteoprotective effect of Herba epimedii (HEP) extract in vivo and in vitro. Evidence-based Complementary and Alternative Medicine . 2005;2(3):353–361. doi: 10.1093/ecam/neh101. - DOI - PMC - PubMed

"VSports app下载" LinkOut - more resources