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. 2020 Jun 28;12(7):1914.
doi: 10.3390/nu12071914.

"V体育官网" Lactobacillus plantarum GKM3 and Lactobacillus paracasei GKS6 Supplementation Ameliorates Bone Loss in Ovariectomized Mice by Promoting Osteoblast Differentiation and Inhibiting Osteoclast Formation

Li-Chan Yang  1 , Shih-Wei Lin  2 , I-Chen Li  2 , Yen-Po Chen  2 , Shih-Yang Tzu  2 , Wei Chou  2 , Chin-Chu Chen  2   3   4   5 , Wen-Chuan Lin  1 , Yen-Lien Chen  2 , Wen-Hsin Lin  1
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VSports手机版 - Lactobacillus plantarum GKM3 and Lactobacillus paracasei GKS6 Supplementation Ameliorates Bone Loss in Ovariectomized Mice by Promoting Osteoblast Differentiation and Inhibiting Osteoclast Formation

Li-Chan Yang et al. Nutrients. .

V体育安卓版 - Abstract

Osteoporosis, an imbalance in the bone-forming process mediated by osteoblasts and the bone-resorbing function mediated by osteoclasts, is a bone degenerative disease prevalent among the aged population. Due to deleterious side effects of currently available medications, probiotics as a potential treatment of osteoporosis is an appealing approach. Hence, this study aims to evaluate the beneficial effects of two novel Lactobacilli strain probiotics on bone health in ovariectomized (OVX) induced osteoporotic mice model and its underlying mechanisms. Forty-five 9-week-old Institute of Cancer Research (ICR) mice underwent either a sham-operation (n = 9) or OVX (n = 36). Four days after the operation, OVX mice were further divided into four groups and received either saline alone, Lactobacillus plantarum GKM3, Lactobacillus paracasei GKS6 or alendronate per day for 28 days. After sacrifice by decapitation, right distal femur diaphysis was imaged via micro-computed tomography (MCT) and parameters including bone volume/tissue volume ratio (BV/TV), trabecular thickness (Tb. Th), trabecular number (Tb. N), trabecular separation (Tb. Sp), and bone mineral density (BMD) were measured. Moreover, GKM3 and GKS6 on RANKL-induced osteoclast formation and osteoblast differentiation using in vitro cultures were also investigated VSports手机版. The results showed that both probiotics strains inhibited osteoporosis in the OVX mice model, with L. paracasei GKS6 outperforming L. plantarum GKM3. Besides this, both GKS6 and GKM3 promoted osteoblast differentiation and inhibited RANKL-induced osteoclast differentiation via the Bone Morphogenetic Proteins (BMP) and RANKL pathways, respectively. These findings suggested that both strains of Lactobacilli may be pursued as potential candidates for the treatment and management of osteoporosis, particularly in postmenopausal osteoporosis. .

Keywords: Lactobacillus paracasei GKS6; Lactobacillus plantarum GKM3; osteoblast; osteoclast; osteoporosis. V体育安卓版.

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"VSports手机版" Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Grape King Bio Inc. provided support in the form of salaries for the authors S. -W. L. , I. ‐C. L. , Y. ‐P. C V体育ios版. , S. ‐Y. T. , W. C. , C. ‐C. C. , Y. ‐L. C. and research materials, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Figures

Figure 1
Figure 1
Body weight changes of mice in different groups. * p < 0.05 compared with ovariectomized (OVX) mice.
Figure 2
Figure 2
Representative Micro Computed Tomography images of Femur in (A) Sham group, (B) OVX untreated group, (C) OVX + GKM3 20.5 mg/kg group, (D) OVX + GKS6 20.5 mg/kg group and (E) OVX + alendronate 2.5 mg/kg group.
Figure 3
Figure 3
Effect of probiotics on osteoblast and osteoclast differentiation. Data are presented as the mean ± SD. For osteoblast differentiation, MG-63 cells were treated with estradiol, GKM3, and GKS6 in 1% FBS medium for 7 days. Gene expression of (A) bone morphogenetic protein-2 (BMP-2), (B) alkaline phosphatase (ALP), and (C) osteocalcin (OCN) on day 7 were determined by Q-PCR. For osteoclast differentiation, RAW264.7 were treated with estradiol, GKM3, and GKS6 in the presence of 50 ng/mL RANKL for 7 days. Gene expression of (D) receptor activator of nuclear factor-κB (RANK), (E) Cellular Oncogene Fos (c-Fos), and (F) tartrate-resistant acid phosphatase (TRAP) on day 7 were determined by Q-PCR. All data were normalized to GADPH. #p < 0.05 compared with control and *p < 0.05 compared with untreated.
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