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. 2019 May;23(5):3597-3602.
doi: 10.1111/jcmm.14260. Epub 2019 Mar 19.

circEPSTI1 regulates ovarian cancer progression via decoying miR-942

Jing Xie  1 , Shufen Wang  1 , Genlin Li  1 , Xia Zhao  1 , Feng Jiang  1 , Jie Liu  1 , Weige Tan  2
Affiliations

"V体育官网入口" circEPSTI1 regulates ovarian cancer progression via decoying miR-942

Jing Xie et al. J Cell Mol Med. 2019 May.

Abstract

Increasing studies show that circular RNAs (circRNAs) play vital roles in tumour progression. But, how circRNAs function in ovarian cancer is mostly unclear. Here, we detected the expression of circEPSTI1 in ovarian cancer and explored the function of circEPSTI1 in ovarian cancer via a series of experiments. Then, we performed luciferase assay and RNA immunoprecipitation (RIP) assay to explore the competing endogenous RNA (ceRNA) function of circEPSTI1 in ovarian cancer VSports手机版. qRT-PCR verified that circEPSTI1 was overexpressed in ovarian cancer. Inhibition of circEPSTI1 suppressed ovarian cancer cell proliferation, invasion but promoted cell apoptosis. Luciferase assays and RIP assay showed that circEPSTI1 and EPSTI1 (epithelial stromal interaction 1) could directly bind to miR-942. And circEPSTI1 could regulate EPSTI1 expression via sponging miR-942. In summary, circEPSTI1 regulated EPSTI1 expression and ovarian cancer progression by sponging miR-942. circEPSTI1 could be used as a biomarker and therapeutic target in ovarian cancer. .

Keywords: EPSTI1; circEPSTI1; circular RNAs; competitive endogenous RNAs; miR-942; ovarian cancer V体育安卓版. .

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
circEPSTI1 is up‐regulated and promotes ovarian cancer progression in vitro A. The expression of circEPSTI1 in 50 paired ovarian cancer tissue (Tumour) and adjacent normal tissues (Normal). B, Cells were transfected with si‐NC or si‐circEPSTI1, and the expression of circEPSTI1 (left) and EPSTI1 (right) were determined by qRT‐PCR analysis. C, CCK‐8 assay was performed to assess cell growth. D, Cell apoptosis was determined 48 h after transfection. E, Transwell assay was performed. Representative images of invaded cells are shown in the left panel, and the results are summarized in the right panel. Original magnification, ×200. **P < 0.01
Figure 2
Figure 2
circEPSTI1 promotes ovarian cancer progression in vivo A. Representative images of xenografts tumour in nude mice are shown in the left panel. The weights of xenograft tumours are summarized in the right panel. B, Representative images of HE stained lung metastatic nodules are shown in the left panel. The number of metastatic nodules was summarized in the right panel. *P < 0.05, **P < 0.01
Figure 3
Figure 3
circEPSTI1 acts as a decoy for miR‐942 A. The levels of U6, GAPDH and circEPSTI1 were assessed by qRT‐PCR in nuclear and cytoplasmic fractions. B, The predicted binding sites of miR‐942 within circEPSTI1 were shown. C, The expression of miR‐942 in 50 paired ovarian cancer tissue (Tumour) and adjacent normal tissues (Normal). D, Luciferase reporter assay of cells co‐transfected with miR‐942 mimics and luciferase reporter containing circEPSTI1 (wt) or mutant construct (mut). E, MS2bp‐MS2bs based RIP assay in cells transfected with MS2bs‐circEPSTI1, MS2bs‐circEPSTI1mt or MS2bs‐Rluc. **P < 0.01
Figure 4
Figure 4
circEPSTI1 regulates EPSTI1 via miR‐942 A. The predicted binding sites of miR‐942 within EPSTI1 3’UTR were shown. B, The expression of EPSTI1 in 50 paired ovarian cancer tissue (Tumour) and adjacent normal tissues (Normal). C, Luciferase reporter assay of cells co‐transfected with miR‐942 mimics and luciferase reporter containing EPSTI1 3′UTR (wt) or mutant construct (mut). D, Cells were transfected as described, and the expression of EPSTI1 was determined by qRT‐PCR. E, Cells were transfected as described, and the expression of EPSTI1 was determined by Western blot (left) and quantified (right). F, RIP assay showing the enrichment of circEPSTI1, EPSTI1 and miR‐942 on Ago2 relative to IgG. G, Cells were transfected as described and RIP assay on Ago2 was performed. H, Cells were transfected as described, and the expression of EPSTI1 was determined by qRT‐PCR. **P < 0.01
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