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. 2018 Jul 27:18:105.
doi: 10.1186/s12935-018-0601-4. eCollection 2018.

miR-145 and miR-497 suppress TGF-β-induced epithelial-mesenchymal transition of non-small cell lung cancer by targeting MTDH

Qi Yin #  1 , Yang Han #  2 , Dongyi Zhu  1 , Zhanxia Li  3 , Shan Shan  4 , Wenjing Jin  5 , Qingchun Lu  1 , Tao Ren  1   4
Affiliations

miR-145 and miR-497 suppress TGF-β-induced epithelial-mesenchymal transition of non-small cell lung cancer by targeting MTDH (V体育官网)

Qi Yin et al. Cancer Cell Int. .

Abstract

Background: MicroRNAs (miRNAs) have been reported to play crucial roles in multiple cancers including non-small cell lung cancer (NSCLC) VSports手机版. Here, we investigated the role of miR-145 and miR-497 in TGF-β-induced epithelial-mesenchymal transition (EMT) process of NSCLC. .

Methods: We performed quantitative real time PCR (qRT-PCR) to detect the expression level of miR-145 and miR-497 in NSCLC cell lines. Then in the presence/absence of TGF-β, we transfected miRNA mimics or inhibitor into A549 and H1299 cells and investigated the role of miR-145 and miR-497 in cell migration and invasion using transwell and wound-healing assay. The regulation role of miR-145 and miR-497 on Metadherin (MTDH) was determined by luciferase assay V体育安卓版. The expression level of MTDH and EMT markers E-cadherin and vimentin were detected on mRNA and protein level. .

Results: In our study, our results showed that miR-145 and miR-497 were downregulated in NSCLC cell lines. Overexpression of miR-145 and miR-497 inhibited TGF-β-induced EMT and suppressed cancer cell migration and invasion, while the opposite results were observed in cells transfected with miR-145 or miR-497 inhibitor. Moreover, the luciferase assay confirmed that miR-145 and miR-497 attenuated MTDH expression by directly binding 3'-UTR of MTDH mRNA and exert the tumor-suppression role V体育ios版. .

Conclusions: Overall, we demonstrated that miR-145 and miR-497 functioned as EMT-suppressor in NSCLC by targeting MTDH, provided new evidence that miR-145 and miR-497 as potential therapeutic targets VSports最新版本. .

Keywords: Epithelial–mesenchymal transition; MTDH; Non-small cell lung cancer; miR-145; miR-497 V体育平台登录. .

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Figures

Fig. 1
Fig. 1
Expression of miR-145 and miR-497 in NSCLC cell lines. a Relative expression of miR-145 was detected by qRT-PCR in NSCLC cell lines and normal cell line HBE. b Relative expression of miR-497 in NSCLC cell lines and HBE cell line was evaluated by qRT-PCR. (**P < 0.05)
Fig. 2
Fig. 2
miR-145 and miR-497 in NSCLC cell migration and invasion. a, b After transfection of miR-145 or miR-497 mimic, cells were treated with or without TGF-β and transwell cell migration and invasion assays were performed. c, d Transfection with miR-145 and miR-497 inhibitor resulted in increased NSCLC cell migration and invasion rate in the presence/absence of TGF-β. Mimic NC or inhibitor NC (CTR) was used as control. (**P < 0.05)
Fig. 3
Fig. 3
Wound-healing assay in NSCLC. a, b Relative migrating areas were evaluated in A549 cells transfected with miR-145/miR-497 mimic or inhibitor. c, d After transfection of miR-145/miR-497 mimic or inhibitor, cells were treated with/without TGF-β and the relative migrating areas of H1299 cell were detected.(**P < 0.05)
Fig. 4
Fig. 4
miR-145 and miR-497 inhibit EMT in NSCLC cells. a, b Western blot analysis for the expression of E-cadherin and vimentin in A549 cells treated with miR-145/miR-497 mimic or inhibitor. c, d The expression level of vimentin in H1299 cells transfected with miR-145/miR-497 mimic or inhibitor. (**P < 0.05)
Fig. 5
Fig. 5
miR-145 and miR-497 directly target MTDH. a, b The potential binding sites of miR-145/miR-497 and MTDH were predicted by bioinformatics tools Starbase v2.0 and Targetscan. c, d Relative luciferase activity in cells co-transfected with wild-type/mutant plasmid and miR-145/miR-497 mimic were evaluated. e–h The expression levels of MTDH in A549 and H1299 cells transfected with miR-145/miR-497 mimic or inhibitor. i, j The mRNA levels of MTDH in A549 and H1299 cells transfected with miR-145/miR-497 mimic or inhibitor. (**P < 0.05)
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