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. 2017:2017:8085325.
doi: 10.1155/2017/8085325. Epub 2017 Dec 27.

"V体育ios版" Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway

Jun-Ming Huang  1 , Yuan Bao  1 , Wei Xiang  1 , Xing-Zhi Jing  1 , Jia-Chao Guo  1 , Xu-Dong Yao  1 , Rui Wang  1 , Feng-Jin Guo  1
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"VSports最新版本" Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway

Jun-Ming Huang et al. Evid Based Complement Alternat Med. 2017.

Abstract (V体育平台登录)

Fat infiltration within the bone marrow is easily observed in some postmenopausal women. Those fats are mainly derived from bone marrow mesenchymal stem cells (BMMSCs). The increment of adipocytes derived from BMMSCs leads to decreased osteoblasts derived from BMMSCs, so the bidirectional differentiation of BMMSCs significantly contributes to osteoporosis. Icariin is the main extractive of Herba Epimedii which is widely used in traditional Chinese medicine. In this experiment, we investigated the effect of icariin on the bidirectional differentiation of BMMSCs through quantitative real-time PCR, immunofluorescence, western blot, and tissue sections in vitro and in vivo. We found that icariin obviously promotes osteogenesis and inhibits adipogenesis through detecting staining and gene expression. Micro-CT analysis showed that icariin treatment alleviated the loss of cancellous bone of the distal femur in ovariectomized (OVX) mice. H&E staining analysis showed that icariin-treated OVX mice obtained higher bone mass and fewer bone marrow lipid droplets than OVX mice VSports手机版. Western blot and immunofluorescence showed that icariin regulates the bidirectional differentiation of BMMSCs via canonical Wnt signaling. This study demonstrates that icariin exerts its antiosteoporotic effect by regulating the bidirectional differentiation of BMMSCs through the canonical Wnt signaling pathway. .

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"VSports app下载" Figures

Figure 1
Figure 1
Icariin inhibits OVX-induced bone loss. (a) Images of the distal femurs were obtained after 6-week intervention (top, coronal view of the metaphyseal region; middle, sagittal view; bottom, transverse view). (b) Analysis of the parameter of the three-dimensional trabecular structure of the distal femur: BV/TV: trabecular bone volume/tissue volume; Tb.N: trabecular number; Tb.Sp: trabecular separation; Tb.Th: trabecular thickness. Data represented as mean ± SD, n = 10. ∗P < 0.05; ∗∗∗∗P < 0.0001.
Figure 2
Figure 2
Icariin inhibits angiogenesis in VOX mouse. (a) Femurs were fixed and then embedded in paraffin. Scale bar: 40x magnification. Images stained with H&E in the distal femurs were obtained. (b) Quantification of N.Adi/Ma.Ar was measured through bone histomorphometry analysis of the selected area shown in (a) by Image-Pro Plus. N.Adi/Ma.Ar: number of adipocytes/the area of the bone marrow. Data represented as mean ± SD, n = 10. ∗P < 0.05; ∗∗∗∗P < 0.0001.
Figure 3
Figure 3
Icariin promotes osteogenesis differentiation and inhibits adipogenesis differentiation of BMMSCs. (a) Effect of icariin treatment on proliferation of BMMSCs at different time points. Data presented as mean ± SD. n = 3. ∗P < 0.05 versus control. (b) Effect of icariin on ALP activity and formation of calcium nodules and lipid droplets. (c) Effect of icariin on mRNA expression of ALP, Runx2, COL1, PPARγ, Fabp4, and adipsin. Data presented as mean ± SD, n = 3.
Figure 4
Figure 4
Icariin activated Wnt signaling pathway in BMMSCs. (a) Images of icariin intervention on expression of P-Gsk3β and β-catenin. (b) Images of icariin and DDK-1 intervention on expression of P-Gsk3β and β-catenin. (c) Immunofluorescence images of icariin and DKK-1 intervention on intracellular localization of β-catenin.
Figure 5
Figure 5
Icariin had an antagonistic effect on DDK-1 in the differentiation of BMMSCs. (a) Effect of icariin and DKK-1 intervention on ALP activity and formation of calcium nodules and lipid droplets. Scale bar: 100x magnification. (b) Effect of icariin and DKK-1 intervention on mRNA expression of ALP, Runx2, COL1, PPARγ, Fabp4, and adipsin. Data presented as mean ± SD, n = 3. ∗P < 0.05.
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