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. 2013 Aug;3(4):241-247.
doi: 10.1016/j.jpha.2012.12.001. Epub 2012 Dec 10.

A simple method for the isolation and purification of resveratrol from Polygonum cuspidatum

Dong-Geng Wang  1 , Wen-Ying Liu  2 , Guang-Tong Chen  1   2
Affiliations

A simple method for the isolation and purification of resveratrol from V体育平台登录 - Polygonum cuspidatum

Dong-Geng Wang et al. J Pharm Anal. 2013 Aug.

Abstract

Resveratrol, a polyphenol compound with strong biological activity, has been widely used in medicine, health products and cosmetic industries. It is also the main active component of Polygonum cuspidatum, a well-known traditional Chinese medicine VSports手机版. We developed a simple and effective method for the preparation of resveratrol from P. cuspidatum. The whole preparative process consisted of reflux extraction, filtering, hydrolyzing, liquid-liquid extraction and eluting. Filtering is to remove non polar or less polar compounds and debris fragments from the extract. Hydrolyzing is to transform polydatin to resveratrol to improve the yield of resveratrol. Eluting is to remove impurities including strong acidic and water-soluble compounds. By acid hydrolysis of glycoside (polydatin), the yield of resveratrol increased about 4-fold. The extraction recovery in different stages was high, and the content of resveratrol in the final product was over 73. 8%. Compared with other methods reported, this technology is eco-friendly, easier to perform, and also has a lower cost. .

Keywords: Polygonum cuspidatum; Purification; Resveratrol; Solvent extraction. V体育安卓版.

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Figures

Fig. 1
Fig. 1
Chemical structures of trans-resveratrol (A) and trans-polydatin (B).
Fig. 2
Fig. 2
Flow chart representative of the extraction and purification processes of resveratrol.
Fig. 3
Fig. 3
HPLC chromatograms of samples in filtration and hydrolyzing. (A) Chromatogram of 95% ethanol extract, (B) and (C) chromatograms of residue and aqueous solution after filtration, (D) chromatograms of aqueous solution after hydrolyzing for 6 h. Peak1 polydatin, 3 resveratrol, 4 emodin, 2 and 5 unknown components.
Fig. 4
Fig. 4
Effect of extract–water ratio (g/mL) on the extraction recovery of resveratrol (n=3).
Fig. 5
Fig. 5
HPLC chromatograms of samples after liquid–liquid extraction. (A) chromatogram of organic phase, and (B) chromatogram of aqueous phase.
Fig. 6
Fig. 6
Effect of pH on resveratrol extraction during the eluting process.
Fig. 7
Fig. 7
HPLC chromatograms of samples after eluting. (A) chromatogram of aqueous phase, and (B) chromatogram of organic phase.
None
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V体育ios版 - References

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