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. 2015 Aug;10(2):525-530.
doi: 10.3892/etm.2015.2555. Epub 2015 Jun 5.

Resveratrol relieves ischemia-induced oxidative stress in the hippocampus by activating SIRT1

Zhuangzhi Meng  1 , Jianguo Li  2 , Honglin Zhao  1 , Haiying Liu  1 , Guowei Zhang  1 , Lingzhan Wang  1 , H E Hu  1 , D I Li  1 , Mingjing Liu  1 , Fulong Bi  1 , Xiaoping Wang  1 , Geng Tian  1 , Qiang Liu  3 , Batu Buren  4
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"V体育ios版" Resveratrol relieves ischemia-induced oxidative stress in the hippocampus by activating SIRT1

Zhuangzhi Meng et al. Exp Ther Med. 2015 Aug.

"V体育官网入口" Abstract

Resveratrol, a naturally occurring phytoalexin, acts as an activator of sirtuin 1 (SIRT1) and has been shown to have a neuroprotective role in various models. Healthy adult male Sprague-Dawley rats were subjected to cerebral ischemia in order to study the protective effect of resveratrol on the brain following ischemia, and to investigate the effects of SIRT1 activation on the hippocampus. Untreated and resveratrol-treated rats were anesthetized prior to undergoing surgery to induce middle cerebral artery occlusion followed by reperfusion. SIRT1 expression was evaluated by immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction, and SIRT1 activity was also evaluated. In addition, terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) and Nissl staining assays were conducted and the levels of reactive oxygen species were determined. It was observed that resveratrol significantly decreased the number of TUNEL-positive cells and increased the expression of SIRT1 mRNA in a dose-dependent manner. This was accompanied by increases in SIRT1 protein expression levels and SIRT1 activity. The results demonstrate the neuroprotective and antioxidant effects of resveratrol against ischemia-induced apoptosis in the rat hippocampus VSports手机版. .

Keywords: brain ischemia; hippocampus; reactive oxygen species; resveratrol; sirtuin 1 V体育安卓版. .

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Figures

Figure 1.
Figure 1.
Representative images for (A, D, G) CV, (B, E, H) SIRT1 and (C, F, I) TUNEL staining in the CA3 hippocampal region. Scale bars: (B, C, E, F, H, I) 50 µm. The box indicates the image positioning of SIRT1 and TUNEL. CV, cresyl violet; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end-labeling; SIRT1, sirtuin 1; CON, control; ISC, ischemia; RSV, resveratrol-10.
Figure 2.
Figure 2.
(A) TUNEL staining results and (B) mean values of ROS production. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end-labeling; ROS, reactive oxygen species; DCF, dichlorofluorescein; Con, control; ISC, ischemia; RSV-5, 5 mg/kg resveratrol; RSV-10, 10 mg/kg resveratrol. *P<0.05, **P<0.01.
Figure 3.
Figure 3.
Effects of resveratrol on the expression levels of SIRT1. (A) Western blotting results, (B) protein expression levels and (C) mRNA expression levels (expressed as relative values, which are mean ± standard deviation values compared with the control). SIRT1, sirtuin 1; Con, control; ISC, ischemia; RSV-5, 5 mg/kg resveratrol; RSV-10, 10 mg/kg resveratrol. *P<0.05, **P<0.01.
Figure 4.
Figure 4.
SIRT1 enzyme activity in rats following ischemia (ISC) and resveratrol treatment, measured in total protein extracts from the hippocampal tissues. Results were normalized to control levels. SIRT1, sirtuin 1; RSV-5, 5 mg/kg resveratrol; RSV-10, 10 mg/kg resveratrol. **P<0.01.
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