Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in VSports app下载. gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2015 Jun;48(3):375-84.
doi: 10.1111/cpr.12185. Epub 2015 Apr 13.

VSports app下载 - Icariin induces osteogenic differentiation of bone mesenchymal stem cells in a MAPK-dependent manner

Yuqiong Wu  1 , Lunguo Xia, Yuning Zhou, Yuanjin Xu, Xinquan Jiang
Affiliations

Icariin induces osteogenic differentiation of bone mesenchymal stem cells in a MAPK-dependent manner

Yuqiong Wu et al. Cell Prolif. 2015 Jun.

Abstract

Objectives: Icariin, a flavonoid isolated from Epimedium pubescens, has previously been identified to exert beneficial effects on preventing bone loss and promoting bone regeneration VSports手机版. However, molecular mechanisms for its anabolic action have, up to now, remained largely unknown. .

Materials and methods: Effects of icariin on cell proliferation and osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs) were systematically evaluated. To characterize underlying mechanisms, its effects on mitogen-activated protein kinase (MAPK) signalling pathways were determined V体育安卓版. .

Results: Results showed that icariin might not have enhanced effects on cell proliferation V体育ios版. However, it seemed to significantly enhance osteogenic differentiation of BMSCs, demonstrated by increasing alkaline phosphatase (ALP) activity and gene expression of collagen type I (Col I), osteocalcin (OCN) and osteopotin (OPN). It was demonstrated that icariin rapidly phosphorylated extracellular signal-regulated kinase (ERK), p38 kinase and c-Jun N terminal kinase (JNK). Furthermore, icariin-stimulated osteogenic effects on BMSCs were dramatically attenuated by treatment with either specific ERK inhibitor of PD98059, p38 inhibitor of SB202190 or JNK inhibitor SP600125. .

Conclusions: These results provide a potential mechanism of anabolic activity of icariin on BMSCs involving ERK, p38 and JNK MAPK pathways VSports最新版本. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
Proliferation and apoptosis of BMSC s treated with icariin. (a) Cytotoxicity evaluation with different concentrations of icariin (μm). (b) Proliferation of BMSCs after treatment of icariin by MTT assay. (c) Cell cycle assay of BMSCs after treatment of icariin. (d, e) Apoptosis assay of BMSCs (*as compared to 0 μm group at each time point, *P < 0.05, ** P < 0.01; n = 3).
Figure 2
Figure 2
Protein and gene expression of osteoblastic markers in BMSC s treated with icariin. (a–d) Real‐time PCR analysis of Runx2, Collagen I, OCN and OPN mRNA in BMSCs treated with icariin (*compared to 0 μm at each time point). (e) Protein expression of Runx2 and OPN at concentration of 20 μm by western blotting assay. (f, g) Densitometric analysis of Runx2 and OPN expression; β‐actin density was used as control (*compared to ratio at 0 min group) (*P < 0.05, **P < 0.01; n = 3).
Figure 3
Figure 3
ALP activity of icariin‐treated BMSCs on day 7. (a) ALP staining of BMSCs after treatment with icariin. (b) ALP activity quantitative assay of icariin‐treated BMSCs measured by pNPP assay (*compared to 0 μm group, *P < 0.05, n = 3).
Figure 4
Figure 4
Effect of icariin on MAPK signalling pathways. (a) Western blotting of total and phosphorylated ERK, p38 and JNK following icariin treatment at 0, 15, 30, 60 and 120 min. (b–d) Densitometric analysis of p‐ERK/ERK, p‐p38/p38 and p‐JNK/JNK expression by western blot analysis (*P < 0.05, **P < 0.01; *compared to phosphorylated protein expression at 0 min).
Figure 5
Figure 5
Effect of ERK , p38 and JNK signalling pathways on osteogenic differentiation of BMSC s treated with or without icariin. (a–c) Effect of PD98059 (20 μm, PD in short), SB202190 (20 μm, SB in short) and SP600125 (20 μm, SP in short) on osteogenic gene expression of BMSCs without or with icariin (0, IC in short respectively) treatment. (d) ALP staining assay. (*P < 0.05, **,## P < 0.01; *compared to 0 μm group, #compared to icariin group).
See this image and copyright information in PMC

References

    1. Cheng KF, Leung KS, Leung PC (2003) Interactions between modern and Chinese medicinal drugs: a general review. Am. J. Chin. Med. 31, 163–169. - PubMed
    1. Guo BL, Wang CL, Xiao PG (1996) Determination of flavonoids and quality evaluation of Sagittate Epimedium (Epimedium sagittatum). China Trad. Herb Drugs 27, 524–526.
    1. Nan H, Ma MH, Nan SS, Xu LL (2009) Antiosteoporotic activity of icariin in ovariectomized rats. Phytomedicine 16, 320–326. - VSports app下载 - PubMed
    1. Mok SK, Chen WF, Lai WP, Leung PC, Wang XL, Yao XS et al (2010) Icariin protects against bone loss induced by oestrogen deficiency and activates oestrogen receptor‐dependent osteoblastic functions in UMR 106 cells. Br. J. Pharmacol. 159, 939–949. - PMC - PubMed
    1. Chen KM, Ge BF, Ma HP, Liu XY, Bai MH, Wang Y (2005) Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells. Pharmazie 60, 939–942. - "VSports手机版" PubMed

Publication types

VSports最新版本 - MeSH terms

Substances

VSports - LinkOut - more resources