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Review
. 2014 May 27:5:149.
doi: 10.3389/fgene.2014.00149. eCollection 2014.

Different miRNA expression profiles between human breast cancer tumors and serum (V体育平台登录)

Jie Zhu  1 , Zhibao Zheng  2 , Jia Wang  3 , Jinhua Sun  4 , Pan Wang  1 , Xianying Cheng  4 , Lun Fu  5 , Liming Zhang  1 , Zuojun Wang  1 , Zhaoyun Li  6
Affiliations
Review

Different miRNA expression profiles between human breast cancer tumors and serum

Jie Zhu et al. Front Genet. .

Abstract (VSports注册入口)

A bunch of microRNAs (miRNAs) have been demonstrated to be aberrantly expressed in cancer tumor tissue and serum. The miRNA signatures identified from the serum samples could serve as potential noninvasive diagnostic markers for breast cancer. The role of the miRNAs in cancerigenesis is unclear. In this study, we generated the expression profiles of miRNAs from the paired breast cancer tumors, normal, tissue, and serum samples from eight patients using small RNA-sequencing. Serum samples from eight healthy individuals were used as normal controls. We identified total 174 significantly differentially expressed miRNAs between tumors and the normal tissues, and 109 miRNAs between serum from patients and serum from healthy individuals. There are only 10 common miRNAs. This suggests that only a small portion of tumor miRNAs are released into serum selectively. Interestingly, the expression change pattern of 28 miRNAs is opposite between breast cancer tumors and serum VSports手机版. Functional analysis shows that the differentially expressed miRNAs and their target genes form a complex interaction network affecting many biological processes and involving in many types of cancer such as prostate cancer, basal cell carcinoma, acute myeloid leukemia, and more. .

Keywords: biomarker; breast cancer; miRNA; serum; tumor V体育安卓版. .

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Figures

Figure 1
Figure 1
Correlation analysis of the global miRNAs expression. (A) The scatter plot of genome-wide miRNA expression between breast cancer serum between normal serum (Pearson r2 = 0.98), (B). The scatter plot of genome-wide miRNA expression between breast cancer tumors between the adjacent normal tissue (Pearson r2 = 0.74).
Figure 2
Figure 2
Interaction network of the 10 common differentially expressed miRNAs and their target genes in breast cancer. Target genes associated with only one miRNA are indicated in dark red. Target genes associated with more than one miRNAs are indicated in pink. The 10 common miRNAs are indicated in blue.
Figure 3
Figure 3
Top 15 GO terms enriched in the up-and down-regulated target genes of the differentially miRNAs.
Figure 4
Figure 4
KEGG pathways enriched in the up-and down-regulated target genes of the differentially miRNAs by DAVID.
Figure 5
Figure 5
miR-1246 and miR-485 molecules bind to the target gene. (A) The alignment between CD59 3′ UTR and miR-1246. (B) The alignment between CD59 3′ UTR and miR-485.
See this image and copyright information in PMC

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