Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in VSports app下载. gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2013 Jul 23;8(7):e68926.
doi: 10.1371/journal.pone.0068926. Print 2013.

Low-intensity pulsed ultrasound accelerates tooth movement via activation of the BMP-2 signaling pathway (VSports最新版本)

Hui Xue  1 , Jun Zheng, Ziping Cui, Xiufeng Bai, Gang Li, Caidi Zhang, Sanhu He, Weihong Li, Shayanne A Lajud, Yinzhong Duan, Hong Zhou
Affiliations

V体育官网 - Low-intensity pulsed ultrasound accelerates tooth movement via activation of the BMP-2 signaling pathway

Hui Xue et al. PLoS One. .

Abstract

The present study was designed to determine the underlying mechanism of low-intensity pulsed ultrasound (LIPUS) induced alveolar bone remodeling and the role of BMP-2 expression in a rat orthodontic tooth movement model. Orthodontic appliances were placed between the homonymy upper first molars and the upper central incisors in rats under general anesthesia, followed by daily 20-min LIPUS or sham LIPUS treatment beginning at day 0. Tooth movement distances and molecular changes were evaluated at each observation point. In vitro and in vivo studies were conducted to detect HGF (Hepatocyte growth factor)/Runx2/BMP-2 signaling pathways and receptor activator of NFκB ligand (RANKL) expression by quantitative real time PCR (qRT-PCR), Western blot and immunohistochemistry VSports手机版. At day 3, LIPUS had no effect on the rat orthodontic tooth movement distance and BMP-2-induced alveolar bone remodeling. However, beginning at day 5 and for the following time points, LIPUS significantly increased orthodontic tooth movement distance and BMP-2 signaling pathway and RANKL expression compared with the control group. The qRT-PCR and Western blot data in vitro and in vivo to study BMP-2 expression were consistent with the immunohistochemistry observations. The present study demonstrates that LIPUS promotes alveolar bone remodeling by stimulating the HGF/Runx2/BMP-2 signaling pathway and RANKL expression in a rat orthodontic tooth movement model, and LIPUS increased BMP-2 expression via Runx2 regulation. .

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

"V体育官网入口" Figures

Figure 1
Figure 1. The methodology for tooth movement and a schematic diagram of LIPUS assembly for cell treatment.
(A). View of rat oral cavity, showing that the upper first molar was moved mesially by a closed coil spring at 0.1N of orthodontic force. (B). The 6 well plate filled with medium was placed in the LIPUS field at a distance of 4 mm, which can generate optimized beam uniformity across the target cell region. The sterilized LIPUS transducer probe was suspended above the culture medium (using a clamp stand) partially immersed in the culture medium. The water bath was maintained at 37°C.
Figure 2
Figure 2. The effect of LIPUS stimulation on rat tooth movement.
The amount of tooth movement in the LIPUS group was significantly greater than the control group on day 5, day 7, and day 14. *Significantly different from corresponding non-stimulation group (P<0.05). Values are shown as the mean ± SD, and n = 6.
Figure 3
Figure 3. Quantitative analysis of BMP-2 expression.
(A). Effects of LIPUS stimulation on BMP-2 positive cells in the periodontium is shown by immunohistochemistry (bar: 20 μm). BMP-2 immunoreactivity was observed on the alveolar bone surface in the periodontium of the tension side in the LIPUS stimulation group (g–j) on days 3, 5, 7, and 14, significantly higher than that (b–e) in the non-stimulation group. (B). The number of BMP-2 positive cells in the LIPUS stimulation group was greater than that in the control group on days 3, 5, 7, and 14. *indicates P<0.05. Values are shown as the mean ± SD, and n = 4.
Figure 4
Figure 4. LIPUS stimulation increased osteoclast number and RANKL expression on the pressure side.
(A). Light micrographs of PDL tissue on the compressed side in rat on days 0, 3, and 7 after LIPUS stimulation. A few osteoclasts (arrowheads) are seen in the PDL proper on day 3 and day 7. #: PDL; &: alveolar bone, scale bar, 20 μm. (B). Osteoclast number was counted in the area indicated in Fig. 4A. The quantified data shown represent the mean ± SD for three rats, and each rat contains four random fields, and all the osteoclasts were verified by a pathologist. LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01. Rat upper first molars were stimulated with or without LIPUS for different time intervals, and RANKL mRNA (C) and protein amount (D, E) increased at 3 and 7 days after LIPUS stimulation than day 0. LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.01; 0 day vs 7 day, P<0.001.
Figure 5
Figure 5. LIPUS stimulation enhanced the BMP-2 signaling pathway gene expression in vitro and in vivo.
(A). hPDL cells were cultured in the presence and absence of daily LIPUS stimulation. BMP-2 mRNA expression was determined using qRT-PCR. (B). BMP-2 protein (ROW 1; 45 kDa) can be detected in the control and LIPUS groups (1: CON; 2: LIPUS). The LIPUS groups showed higher expression of BMP-2 from day 5. (C). Quantification of BMP-2 protein expression in the LIPUS stimulation group was greater than that in the control group on days 5, 7, and 14. *indicates P<0.05. (D–F). Rat upper first molars were stimulated with or without LIPUS for different time intervals, and HGF, Runx2, BMP-2 were determined by qRT-PCR and Western blot, respectively. The data indicated that LIPUS increased HGF, Runx2, and BMP-2 mRNA (D: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01) and protein expression (E, F: LIPUS stimulation 0 day vs LIPUS stimulation 3 day, P<0.05; 0 day vs 7 day, P<0.01) in vivo. The data are the mean ± SD of three separate experiments.
Figure 6
Figure 6. LIPUS stimulation increased BMP-2 expression mediated by Runx2.
(A). hPDL cells were incubated with HGF for 24 h, and BMP-2 mRNA was examined by qRT-PCR. (B and C) hPDL cells were incubated with HGF for 48 h, and BMP-2 protein amounts were detected by Western blotting. The data shows that HGF significantly increased BMP-2 expression. (D). Cells were transfected with Runx2 siRNA for 24 h followed by stimulation with LIPUS for 5 days, and BMP-2 mRNA expression was examined by qRT-PCR. (E and F) hPDL cells were transfected with Runx2 siRNA for 5 days, and BMP-2 protein expression was examined by Western blot. Transfection of cells with Runx2 siRNA reduced LIPUS-increased BMP-2 expression. *P<0.05 (**P<0.01) as compared with the control group.
See this image and copyright information in PMC

References

    1. Spadaro JA (1997) Mechanical and electrical interactions in bone remodeling. Bioelectromagnetics 18: 193–202. - PubMed (VSports在线直播)
    1. Kobayashi Y, Takagi H, Sakai H, Hashimoto F, Mataki S, et al. (1998) Effects of local administration of osteocalcin on experimental tooth movement. Angle Orthod 68: 259–266. - PubMed (VSports在线直播)
    1. Iino S, Sakoda S, Ito G, Nishimori T, Ikeda T, et al... (2007) Acceleration of orthodontic tooth movement by alveolar corticotomy in the dog. Am J Orthod Dentofacial Orthop 131: 448 e441–448. - PubMed
    1. Kim SJ, Park YG, Kang SG (2009) Effects of Corticision on paradental remodeling in orthodontic tooth movement. Angle Orthod 79: 284–291. - PubMed (V体育官网)
    1. Lee W, Karapetyan G, Moats R, Yamashita DD, Moon HB, et al. (2008) Corticotomy-/osteotomy-assisted tooth movement microCTs differ. J Dent Res 87: 861–867. - PubMed

Publication types (VSports注册入口)

Substances

LinkOut - more resources