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. 2012;7(9):e44568.
doi: 10.1371/journal.pone.0044568. Epub 2012 Sep 7.

High incidence of multiple viral infections identified in upper respiratory tract infected children under three years of age in Shanghai, China

Guocui Zhang  1 , Yunwen Hu, Hongping Wang, Lu Zhang, Yixi Bao, Xiaoming Zhou
Affiliations

High incidence of multiple viral infections identified in upper respiratory tract infected children under three years of age in Shanghai, China

Guocui Zhang et al. PLoS One. 2012.

Abstract (VSports最新版本)

Background: Upper respiratory tract infection (URTI) is a major reason for hospitalization in childhood. More than 80% of URTIs are viral. Etiological diagnosis of URTIs is important to make correct clinical decisions on treatment methods VSports手机版. However, data for viral spectrum of URTIs are very limited in Shanghai children. .

Methods: Nasopharyngeal swabs were collected from a group of 164 children aged below 3 years who were hospitalized due to acute respiratory infection from May 2009 to July 2010 in Shanghai V体育安卓版. A VRDAL multiplex PCR for 10 common respiratory viruses was performed on collected specimens compared with the Seeplex® RV15 ACE Detection kit for 15 respiratory viruses. .

Results: Viruses were detected in 84 (51. 2%) patients by VRDAL multiplex PCR, and 8 (4. 9%) of cases were mixed infections. Using the Seeplex® RV15 ACE Detection kit, viruses were detected in 129 (78. 7%) patients, 49 (29. 9%) were co-infected cases. Identified viruses included 37 of human rhinovirus (22. 6% of cases), 32 of influenza A virus (19. 5%), 30 of parainfluenzavirus-2 (18. 3%), 23 of parainfluenzavirus-3 (14 V体育ios版. 0%), 15 of human enterovirus (9. 1%), 14 each of parainfluenzavirus-1, respiratory syncytial virus B and adenovirus (8. 5%), 8 of coronavirus 229E/NL63 (4. 9%), 6 of human bocavirus (3. 7%), 5 each of influenza B virus and respiratory syncytial virus A (3. 0%), 3 of parainfluenzavirus-4 (1. 8%), 2 of coronavirus OC43/HKU1 (1. 2%), and 1 human metapneumovirus (0. 6%). .

Conclusions: A high frequency of respiratory infections (78. 7%) and co-infections (29. 9%) was detected in children with acute respiratory infection symptoms in Shanghai. The Seeplex® RV15 ACE detection method was found to be a more reliable high throughput tool than VRDAL method to simultaneously detect multiple respiratory viruses VSports最新版本. .

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"VSports在线直播" Conflict of interest statement

Competing Interests: The authors are served as research employees in Shanghai Public Health Clinical Center, China. This does not alter the authors’ adherence to all the PLoS ONE policies on sharing data and materials V体育平台登录.

Figures

Figure 1
Figure 1. One example analyzed both by Seeplex® RV15 and VRDAL multiplex PCRs.
lane “M”: molecular PCR marker, size of each bands were annotated in left; “IC”: 850 bp internal positive control, all presented in lane 1a–1c; “1”: one sample (A2-1319T) analyzed by Seeplex® RV15 method; lane “1a”, “1b”, “1c”: three individual reaction groups, showed a quartile infections included PIV2(264 bp, 1a), PIV3(189 bp, 1a), FluA(206 bp, 1b) and HboV(579 bp, 1c); “2”: same sample analyzed by VRDAL method; lane “2a”, “2b”, “2c”: three individual reaction groups, showed single FluA infection detected (196 bp, 2a) in lane 2a with negative results detected both in lane 2b and 2c.
See this image and copyright information in PMC

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