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. 2012 Feb;3(2):281-286.
doi: 10.3892/ol.2011.483. Epub 2011 Nov 15.

Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors

Yusuke Nakano  1 , Toshiyuki Sumi, Masanari Morishita, Takeshi Fukuda, Hiroyuki Nobeyama, Hiroyuki Yoshida, Yoshinari Matsumoto, Tomoyo Yasui, Osamu Ishiko
Affiliations

V体育2025版 - Mitotic arrest deficiency 2 induces carcinogenesis in mucinous ovarian tumors

Yusuke Nakano (V体育安卓版) et al. Oncol Lett. 2012 Feb.

Abstract

Mitotic arrest deficiency 2 (MAD2) is a key component of the mitotic spindle checkpoint pathway. A compromised mitotic spindle checkpoint results in an abnormal number of chromosomes. This is referred to as chromosomal instability, and has been reported in most types of human cancer VSports手机版. The aim of this study was to examine the expression of MAD2 in mucinous ovarian tumors exhibiting varying degrees of malignancy. We reviewed 128 cases of mucinous ovarian tumors initially treated at Osaka City University Medical School Hospital, Japan. Tumor samples were obtained following surgery. The cases were divided into three groups: benign (group B; n=30), borderline malignant (group BM; n=55) and malignant (group M; n=43). MAD2 expression was examined in paraffin-embedded sections using the avidin-biotin peroxidase complex method. Results showed MAD2 expression to be significantly greater in group M compared to groups B and BM (P<0. 05). In addition, there was a moderate correlation between MAD2 expression and the degree of malignancy (r=0. 51, P<0. 05). However, when the samples in group M were classified according to a low or high expression of MAD2, no difference was observed in terms of overall survival. These findings suggest that the overexpression of MAD2 may be correlated to carcinogenesis in mucinous ovarian tumors. .

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Figures

Figure 1
Figure 1
Immunohistochemical staining of MAD2 in mucinous ovarian tumors. (A) Group M, score 12; (B) Group BM, score 3; (C) Group B, score 0 (A-C, H&E; magnification, ×400). MAD2 was expressed in the nuclei of the tumor cells.
Figure 2
Figure 2
Weighted score for MAD2 in mucinous ovarian tumors is shown. MAD2 expression was significantly greater in group M than in groups B and BM. *P<0.05 (Mann-Whitney U test). MAD2 expression tended to be higher in BM than in group B (P=0.064, Mann-Whitney U test). Thus, there was a moderate correlation between MAD2 expression and the degree of malignancy in mucinous ovarian tumors (r=0.51, P<0.05, Spearman’s rank correlation coefficient).
Figure 3
Figure 3
Overall survival according to FIGO criteria in group M. A total of 43 cases in group M were sub-classified into stage I (n=25), stage II (n=8) and stage III/IV (n=10) according to FIGO criteria. The overall survival for cases with stage III/IV was significantly shorter than that for cases with stage I and stage II (P<0.05, Kaplan-Meier and log-rank tests).
Figure 4
Figure 4
Overall survival according to the grade of MAD2 expression. The mean weighted scores in 43 cases in group M was 7.6. These cases were sub-classified into low and high expression groups according to the weighted score. The low expression group had weighted scores from 2 to 6, and the high expression group had weighted scores from 8 to 12. There were 19 cases with a low expression and 24 with a high expression. No significant difference was observed between the low and high expression groups in terms of overall survival (P=0.903, Kaplan-Meier and log-rank tests).
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