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. 2011 May;96(5):1409-14.
doi: 10.1210/jc.2010-1812. Epub 2011 Feb 2.

A resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal

Husam Ghanim  1 , Chang Ling Sia, Kelly Korzeniewski, Teekam Lohano, Sanaa Abuaysheh, Anuritha Marumganti, Ajay Chaudhuri, Paresh Dandona
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A resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal

"V体育官网入口" Husam Ghanim et al. J Clin Endocrinol Metab. 2011 May.

Abstract

Background: High-fat, high-carbohydrate (HFHC) meals are known to induce oxidative and inflammatory stress, an increase in plasma endotoxin concentrations, and an increase in the expression of suppressor of cytokine signaling-3 (SOCS-3) VSports手机版. .

Hypothesis: The intake of a nutritional supplement containing resveratrol and muscadine grape polyphenols reduces HFHC meal-induced oxidative and inflammatory stress and stimulates the activity of the antioxidant transcription factor, NF-E2-related factor-2 (Nrf-2), and its downstream targets. V体育安卓版.

Methods: Ten normal, healthy subjects were given a 930-kcal HFHC meal either with placebo or with the supplement. Indices of oxidative stress, inflammation, Nrf-2 binding activity, the concentrations of endotoxin (lipopolysaccharide) and lipoprotein binding protein (LBP), and the expression of toll-like receptor 4 (TLR-4), CD14, IL-1β, TNFα, SOCS-3, Keap-1, NAD(P)H:quinone oxidoreductase-1 (NQO-1), and GST-P1 were measured. V体育ios版.

Results: The intake of the supplement suppressed the meal-induced elevations of plasma endotoxin and LBP concentrations, the expression of p47(phox), TLR-4, CD14, SOCS-3, IL-1β, and Keap-1, while enhancing Nrf-2 binding activity and the expression of NQO-1 and GST-P1 genes. VSports最新版本.

Conclusion: A supplement containing resveratrol and muscadine polyphenols suppresses the increase in oxidative stress, lipopolysaccharide and LBP concentrations, and expression of TLR-4, CD14, IL-1β and SOCS-3 in mononuclear cells after an HFHC meal V体育平台登录. It also stimulates specific Nrf-2 activity and induces the expression of the related antioxidant genes, NQO-1 and GST-P1. These results demonstrate the acute antioxidant and antiinflammatory effects of resveratrol and polyphenolic compounds in humans in the postprandial state. .

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Figures

Fig. 1.
Fig. 1.
Change from baseline (%) in oxidative stress markers. A, Representative Western blot and protein content of p47phox (B), Keap-1 (E) and NQO1 (F) in total MNC lysates. C and D, Representative shifted and supershifted (SS) band of Nrf-2 binding to specific ARE site in NQO-1 promoter using EMSA in MNC nuclear extracts before and after intake of 930-calorie HFHC meal plus placebo (HFHC + plcb) or a resveratrol and polyphenols supplement (HFHC + Supp) in 10 normal, healthy subjects in a crossover design. Quality controls for EMSA include incubating a 5-h sample from HFHC + Plcb (Plcb) with Nrf-2 antibody (Nrf-2 Ab), cold Nrf-2 oligonucleotide (S cold oligos), or nonspecific oct-1 oligonucleotide (NS cold oligos). Samples were collected before and 1, 3, and 5 h after intake. Data are expressed as mean ± se. *, Placebo + supplement, P < 0.05 compared with baseline by RMANOVA; #, P < 0.05 between groups by two-way RMANOVA.
Fig. 2.
Fig. 2.
Change in LBP (A) concentrations by immunolinked assay, mRNA expression of IL-1β (B) by real time PCR and immunoblotting representative gel (C) for the protein content of SOCS-3 (D), CD14 (E), and TLR4 (F) in MNC before and after intake of 930-calorie HFHC meal plus placebo or a resveratrol and polyphenols supplement in 10 normal healthy subjects in a crossover design. Samples were collected before and at 1, 3, and 5 h after meal intake. Data are represented as mean ± se. *, Placebo + supplement, P < 0.05 compared with baseline by RMANOVA; #, P < 0.05 between groups by two-way RMANOVA.
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References

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