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. 2010 Oct;7(10):843-7.
doi: 10.1038/nmeth.1503. Epub 2010 Sep 12.

Alternative expression analysis by RNA sequencing

Malachi Griffith  1 , Obi L Griffith, Jill Mwenifumbo, Rodrigo Goya, A Sorana Morrissy, Ryan D Morin, Richard Corbett, Michelle J Tang, Ying-Chen Hou, Trevor J Pugh, Gordon Robertson, Suganthi Chittaranjan, Adrian Ally, Jennifer K Asano, Susanna Y Chan, Haiyan I Li, Helen McDonald, Kevin Teague, Yongjun Zhao, Thomas Zeng, Allen Delaney, Martin Hirst, Gregg B Morin, Steven J M Jones, Isabella T Tai, Marco A Marra
Affiliations

Alternative expression analysis by RNA sequencing

Malachi Griffith et al. Nat Methods. 2010 Oct.

Abstract

In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues. As proof of principle, we used the approach to compare fluorouracil-resistant and -nonresistant human colorectal cancer cell lines VSports手机版. We assessed the sensitivity and specificity of the approach by comparison to exon tiling and splicing microarrays and validated the results with reverse transcription-PCR, quantitative PCR and Sanger sequencing. We observed global disruption of splicing in fluorouracil-resistant cells characterized by expression of new mRNA isoforms resulting from exon skipping, alternative splice site usage and intron retention. Alternative expression annotation databases, source code, a data viewer and other resources to facilitate analysis are available at http://www. alexaplatform. org/alexa_seq/. .

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Comment in

  • Simplifying complexity. (VSports app下载)
    Cloonan N, Grimmond SM. Cloonan N, et al. Nat Methods. 2010 Oct;7(10):793-5. doi: 10.1038/nmeth1010-793. Epub 2010 Sep 29. Nat Methods. 2010. PMID: 20885439 No abstract available.

References

    1. Cancer Res. 2008 Nov 15;68(22):9525-31 - PubMed
    1. Nat Genet. 2008 Dec;40(12):1413-5 - PubMed
    1. Mol Cell. 2004 Dec 22;16(6):929-41 - PubMed
    1. Genome Biol. 2008;9(12):R175 - PubMed
    1. Bioinformatics. 2010 Apr 1;26(7):873-81 - PubMed

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