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. 2009 May;6(5):315-6.
doi: 10.1038/nmeth.f.248. Epub 2009 Apr 6.

Massively parallel exon capture and library-free resequencing across 16 genomes (V体育官网)

Emily H Turner, Choli Lee, Sarah B Ng, Deborah A Nickerson, Jay Shendure

Massively parallel exon capture and library-free resequencing across 16 genomes (VSports最新版本)

V体育安卓版 - Emily H Turner et al. Nat Methods. 2009 May.
No abstract available

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Figures

Figure 1
Figure 1. Optimizations yield reduced representational and allelic bias
(a) The uniformity of capture across 55,000 or 13,000 targeted exons is illustrated, estimated by the relative depth of sequence coverage of each target. (b,c) Sequencing-based allele ratios at positions of common variation are plotted for previously described (b) and current (c) protocols. Each point represents a position where targeted resequencing data from a 55,000-plex amplification intersects with a HapMap genotype for this individual (NA12248). The allele ratio is the frequency with which the reference allele was observed in sequence data. Genotypes are indicated according to HapMap data.
Figure 2
Figure 2. Schematic of MIP-based exon capture and direct resequencing
Thousands of MIP precursors can be cost-effectively produced by release from programmable arrays. Each MIP is an oligonucleotide that includes a common linker (green) flanked by target-specific sequences (yellow) that hybridize immediately upstream and downstream of its target. Addition of polymerase and ligase results in copying of targets and conversion to a circular form. Illumina adaptors (black) are appended by a multi-template, inverse PCR reaction, resulting in amplicons that can be directly sequenced. All 76 bp sequencing reads (grey arrows) include 20 bases of targeting arm sequence and an additional 56 bases of within-target sequence.
See this image and copyright information in PMC

References

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