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. 2008 Oct;41(5):803-12.
doi: 10.1111/j.1365-2184.2008.00542.x.

Identification of osteo-adipo progenitor cells in fat tissue (V体育官网入口)

Y F Lin  1 , W Jing, L Wu, X Y Li, Y Wu, L Liu, W Tang, J Long, W D Tian, X M Mo
Affiliations

Identification of osteo-adipo progenitor cells in fat tissue

Y F Lin et al. Cell Prolif. 2008 Oct.

Abstract

Objectives: In this study, a group of cells that expressed both osteogenic and adipogenic characters was identified from murine adipose stromal cells VSports手机版. .

Materials and methods: These cells could be enriched in the Sca-1-1 population and express both osteogenic and adipogenic genes. Osteogenic induction enhanced expression of osteogenic genes and inhibited expression of adipogenic genes, while adipogenic induction enhanced expression of adipogenic genes and inhibited expression of osteogenic genes. These cells have been called osteo-adipo progenitors (OAPs) V体育安卓版. .

Results: OAPs expressed transcription factor runt-related transcription factor 2 (RUNX2) and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) proteins in cytoplasm V体育ios版. When OAPs were cultured in adipogenic medium, PPAR-gamma moved to the nucleus and the cells differentiated into adipocytes, while the RUNX2 remained in the cytoplasm. In contrast, when OAPs were cultured in osteogenic medium, RUNX2 moved to the nucleus and the cells differentiated to osteocytes, while the PPAR-gamma remained in the cytoplasm. .

Conclusions: These experiments suggest that osteoblasts and adipocytes share a common predecessor, the OAP, in murine adipose stromal cells. VSports最新版本.

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Figures

Figure 1
Figure 1
ASCs migrated from small fat tissue sample (a). Mineralized nodules of cells were observed using alizarin red‐S staining, when adipose stromal cells (ASCs) were cultured in osteogenic medium for 2 weeks (b). After 2 weeks adipogenic induction, the accumulation of lipid was observed by Oil Red O staining (c). After chondrogenic induction and pellet culture, toluidine blue staining of ASC pellets proved the cells to have differentiated into chondrocytes; most cells were surrounded by proteoglycans (d).
Figure 2
Figure 2
RT‐PCR analysis of PPAR‐γ, CEBP‐α, LPL, RUNX2, BSP, TAZ and OSX performed for adipogenesis. Day 0 indicates adipose stromal cells (ASCs) cultured in control medium; days 1, 3 and 7 indicate ASCs cultured in adipogenic medium (a). RT‐PCR analysis of PPAR‐γ, CEBP‐α, LPL, RUNX2, BSP, TAZ and OSX for osteogenesis, day 0 meaning ASCs cultured in control medium, and days 1, 3 and 7 indicating ASCs cultured in osteogenic medium (b). The results showed that the ASCs expressed PPAR‐γ, CEBP‐α, LPL, RUNX2, BSP, TAZ and OSX, even without induction. Osteogenic induction enhanced expression of osteogenic genes and inhibited expression of adipogenic genes, while adipogenic induction enhanced expression of adipogenic genes and inhibited expression of osteogenic genes.
Figure 3
Figure 3
By FACS, ASCs could be divided into two populations: a large population Sca‐1+ and a small population Sca‐1− (a). RT‐PCR results showed that the large population expressed adipogenic genes such as PPAR‐γ, CEBP‐α and LPL but were negative for osteogenic genes such as RUNX2, BSP, TAZ and OSX. The small population expressed both adipogenic and osteogenic genes, RUNX2, BSP, TAZ, OSX, PPAR‐γ, CEBP‐α and LPL (b).
Figure 4
Figure 4
Immunofluorecsence staining performed to identify osteogenic and adipogenic cells of adipose stromal cells (ASCs). Among ASCs, a small group of cells expressed both RUNX2 and PPAR‐γ simultaneously, in the cytoplasm of the same cell (Fig. 4, uninduction). When ASCs were transferred to adipogenic medium, PPAR‐γ was detected in nuclei and cytoplasm of cells while RUNX2 existed only in the cytoplasm (Fig. 4, adipogenesis). In comparison, when the ASCs were cultured in osteogenic medium, RUNX2 was detected in nuclei and cytoplasm of cells, while PPAR‐γ remained in the cytoplasm (Fig. 4, osteogenesis).
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