Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. VSports app下载.

Https

The site is secure V体育官网. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. .

. 2007 Sep 26:4:20.
doi: 10.1186/1476-9255-4-20.

Induction of cystine/glutamate transporter in bacterial lipopolysaccharide induced endotoxemia in mice

Kumiko Taguchi #  1 , Michiko Tamba #  1 , Shiro Bannai  1 , Hideyo Sato  2
Affiliations

Induction of cystine/glutamate transporter in bacterial lipopolysaccharide induced endotoxemia in mice

Kumiko Taguchi et al. J Inflamm (Lond). .

Abstract

Background: Cystine/glutamate transporter, system xc-, contributes to the maintenance of intracellular glutathione levels and the redox balance in the extracellular space. The main component of the transporter, xCT, is known to be strongly induced by various stimuli like oxidative stress in mammalian cultured cells VSports手机版. We examined the expression of xCT mRNA in vivo in the experimental endotoxemia. .

Methods: Northern blot analysis and in situ hybridization were used to investigate the expression of xCT mRNA in the tissues of the mice exposed to bacterial lipopolysaccharide (LPS). V体育安卓版.

Results: Northern blot analysis revealed that xCT mRNA was constitutively expressed in the brain, thymus, and spleen, and that the expression of xCT mRNA was strongly up-regulated in thymus and spleen by the administration of a sublethal dose of LPS V体育ios版. In addition to brain, thymus, and spleen, xCT mRNA was detected also in the bronchiolar epithelium of the lung by the administration of the lethal dose of LPS. .

Conclusion: xCT is induced in some specific tissues by the administration of LPS VSports最新版本. The results suggest that cystine/glutamate transporter plays an important role under the inflammatory conditions. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
Tissue distribution of xCT and 4F2hc mRNA in mice injected with or without LPS. Mice were intraperitoneally injected with saline (-) or LPS 0.5 mg/kg LPS (+), and tissues were isolated 8 h after administration. Total RNA was extracted and Northern blot analysis was performed using DIG-labeled RNA probes.
Figure 2
Figure 2
Time course of expression of xCT and 4F2hc mRNA in thymus and spleen of the mice injected with LPS. Mice were intraperitoneally injected with 0.5 mg/kg LPS, and thymus and spleen were isolated at the indicated time points. Total RNA was extracted and Northern blot analysis was performed using DIG-labeled RNA probes.
Figure 3
Figure 3
Dose-dependent expression of xCT and 4F2hc mRNA in thymus and spleen of the mice injected with LPS. Mice were intraperitoneally injected with LPS at the dose indicated, and thymus and spleen were isolated after 8 h. Total RNA was extracted and Northern blot analysis was performed using DIG-labeled RNA probes.
Figure 4
Figure 4
Expression of xCT mRNA in thymus, spleen and lung of the mice injected with lethal dose of LPS by Northern blot analysis. Mice were intraperitoneally injected with saline, 0.5, or 160 mg/kg LPS, and the tissues were isolated 5 h after administration. Total RNA was extracted and Northern blot analysis was performed using DIG-labeled RNA probes.
Figure 5
Figure 5
Expression of xCT mRNA in thymus of the mice injected with a lethal dose of LPS by nonisotopic in situ hybridization. Mice were intraperitoneally injected with saline (A, B) or 160 mg/kg (C, D), and the thymus was isolated after 8 h. Adjacent sections were hybridized with DIG-labeled antisense (A, C) or sense (B, D) probes for xCT. Magnifications: ×50.
Figure 6
Figure 6
Expression of xCT mRNA in spleen of the mice injected with a lethal dose of LPS by nonisotopic in situ hybridization. Mice were intraperitoneally injected with saline (A, B) or 160 mg/kg (C, D), and the spleen was isolated after 8 h. Adjacent sections were hybridized with DIG-labeled antisense (A, C) or sense (B, D) probes for xCT. Magnifications: ×50.
Figure 7
Figure 7
Expression of xCT mRNA in lung of the mice injected with a lethal dose of LPS by nonisotopic in situ hybridization. Mice were intraperitoneally injected with saline (C) or 160 mg/kg (A, B, D), and lung tissue was isolated after 8 h. Adjacent sections were hybridized with DIG-labeled antisense (A-C) or sense (D) probes for xCT. B is a magnification of the boxed region in A. Magnifications: ×50 (A, C, D); ×200 (B).
See this image and copyright information in PMC

References

    1. Raetz CR, Whitfield C. Lipopolysaccharide endotoxin. Annu Rev Biochem. 2002;71:635–700. doi: 10.1146/annurev.biochem.71.110601.135414. - DOI - PMC - PubMed
    1. Cohen J. The immunopathogenesis of sepsis. Nature. 2000;420:885–891. doi: 10.1038/nature01326. - DOI - PubMed
    1. Bannai S, Kitamura E. Transport interaction of L-cystine and Lglutamate in human diploid fibroblasts in culture. J Biol Chem. 1980;255:2372–2376. - PubMed
    1. Bannai S, Tateishi N. Role of membrane transport in metabolism and function of glutathione in mammals. J Membr Biol. 1986;89:1–8. doi: 10.1007/BF01870891. - DOI - PubMed
    1. Bannai S, Sato H, Ishii T, Sugita Y. Induction of cystine transport activity in human fibroblasts by oxygen. J Biol Chem. 1989;264:18480–18484. - PubMed

LinkOut - more resources