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. 2003 Apr 7;88(7):1101-4.
doi: 10.1038/sj.bjc.6600869.

"V体育官网入口" Quantitative analysis of macrophage inhibitory cytokine-1 (MIC-1) gene expression in human prostatic tissues

T Nakamura  1 , A Scorilas, C Stephan, G M Yousef, G Kristiansen, K Jung, E P Diamandis
Affiliations

Quantitative analysis of macrophage inhibitory cytokine-1 (MIC-1) gene expression in human prostatic tissues (VSports最新版本)

T Nakamura (VSports在线直播) et al. Br J Cancer. .

Abstract

Macrophage inhibitory cytokine-1 (MIC-1) gene is a member of transforming growth factor-beta superfamily and was reported to be highly overexpressed in human prostate cancer using microarray technology VSports手机版. The aim of this study was to evaluate the quantitative expression of MIC-1 in malignant and benign prostate tissues and to associate expression levels with clinicopathological parameters of prostate cancer. Matched (paired) prostatic tissue samples from the cancerous and noncancerous parts of the same prostates were obtained from 66 patients who underwent radical prostatectomy. Quantitative RT-PCR was performed using SYBR Green I on the Roche LightCycler system. Macrophage inhibitory cytokine-1 gene overexpression in cancerous tissues was observed in 88% of cases, compared to noncancerous tissues (P<0. 001). The expression level of MIC-1 in cancerous tissues was significantly higher than in noncancerous tissue (P<0. 001). Higher expression of MIC-1 gene was significantly associated with higher Gleason score (P=0. 004). The expression of the MIC-1 gene in prostate cancer is significantly higher than in noncancerous tissues, especially in more aggressive forms of the disease (Gleason score>5). This is in contrast to prostate-specific antigen that is downregulated in higher-grade tumours. The upregulation of MIC-1 in prostate cancer and in advanced and more aggressive prostatic tumours suggests that MIC-1 protein should be evaluated as a potential diagnostic and prognostic biomarker. .

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Figures

Figure 1
Figure 1
MIC-1 mRNA expression as arbitrary units shown for 66 patients. The black box represents the level in cancerous tissue and the connected white box the respective level of the nonmalignant tissue of the same patient. The P-value was calculated by McNemar test.
Figure 2
Figure 2
MIC-1 mRNA expression in cancerous and noncancerous prostatic tissues. The horizontal lines indicate the median. The P-value was calculated by the Mann–Whitney U-test.
See this image and copyright information in PMC

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