Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The . gov means it’s official VSports app下载. Federal government websites often end in . gov or . mil. Before sharing sensitive information, make sure you’re on a federal government site. .

Https

The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely. V体育官网.

. 2002 Dec 1;102(4):338-42.
doi: 10.1002/ijc.10719.

"VSports" Cytoplasmic and/or nuclear accumulation of the beta-catenin protein is a frequent event in human osteosarcoma

Rex C Haydon  1 , Andrea Deyrup, Akira Ishikawa, Robert Heck, Wei Jiang, Lan Zhou, Tao Feng, David King, Hongwei Cheng, Benjamin Breyer, Terrance Peabody, Michael A Simon, Anthony G Montag, Tong-Chuan He
Affiliations

Cytoplasmic and/or nuclear accumulation of the beta-catenin protein is a frequent event in human osteosarcoma

Rex C Haydon et al. Int J Cancer. .

Abstract

The molecular events that precede the development of osteosarcoma, the most common primary malignancy of bone, are unclear, and concurrent molecular and genetic alterations associated with its pathogenesis have yet to be identified. Recent studies suggest that activation of beta-catenin signaling may play an important role in human tumorigenesis. To investigate the potential role of beta-catenin deregulation in human osteosarcoma, we analyzed a panel of 47 osteosarcoma samples for beta-catenin accumulation using immunohistochemistry. Potential activating mutations were investigated by sequencing exon 3 of the beta-catenin gene in genomic DNA isolated from tumor samples. Our findings revealed cytoplasmic and/or nuclear accumulation of beta-catenin in 33 of 47 samples (70. 2%); however, mutation analysis failed to detect any genetic alterations within exon 3, suggesting that other regulatory mechanisms may play an important role in activating beta-catenin signaling in osteosarcoma. In our survival analysis, beta-catenin deregulation conferred a hazard ratio of 1. 05, indicating that beta-catenin accumulation does not appear to be of prognostic value for osteosarcoma patients. When analyzed against other clinicopathologic parameters, beta-catenin accumulation correlated only with younger age at presentation (26. 4 vs. 39. 8 years). Nevertheless, our results demonstrate that the deregulation of beta-catenin signaling is a common occurrence in osteosarcoma that is implicated in the pathogenesis of osteosarcoma VSports手机版. .

PubMed Disclaimer

Figures

Figure 1
Figure 1
Representative immunohistochemical analyses of β-catenin in human osteosarcoma. Three cases of osteosarcoma were selected and 4 µm sections prepared. Immunohistochemical staining was performed using an anti-β-catenin antibody (see Material and Methods). Immunostaining results were graded according to the location and intensity of the signal. Slides were examined at ×40 magnification under a light microscope. Negative specimens had no nuclear/cytoplasmic staining, though many had isolated membranous staining (top row). Positive samples showed significant staining within the nucleus (middle row) and/or cytoplasm (bottom row). Control staining was performed in the same conditions except that a mouse IgG was used. After immunostaining, each section was counterstained with light green to reveal cellular structures. HE staining was also performed to reveal the histology of the selected samples. See text for details. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]
Figure 2
Figure 2
Mutational analyses of the exon 3 region of the β-catenin gene in human osteosarcoma. Genomic DNA was purified from the tumor regions of paraffin-embedded sections of the selected samples. Exon 3 of the β-catenin gene was amplified by PCR and sequenced using the USB ThermoSequenase kit (see Material and Methods). Representative DNA sequences from 5 samples are shown. Tumor samples derived from patients 1, 3 and 4 exhibited significant cytoplasmic accumulation of β-catenin protein; the tumor sample from patient 2 was notable for marked nuclear accumulation of this protein, whereas the tumor sample from patient 3 exhibited strong nuclear and cytoplasmic staining of the β-catenin protein. Except for the specimen from patient 4 (from pulmonary metastatic tumors), all other samples were primary tumors. The frequently mutated GSK3β phosphorylation sites within exon 3 reported in other types of human cancer are indicated.
See this image and copyright information in PMC

References

    1. Mirra JM, Picci P, Gold RH. Bone tumors: clinical, radiologic, and pathologic correlations. Philadelphia: Lea & Febiger; 1989.
    1. Glasser DB, Lane JM, Huvos AG, Marcove RC, Rosen G. Survival, prognosis, and therapeutic response in osteogenic sarcoma. The Memorial Hospital experience. Cancer. 1992;69:698–708. - "V体育官网" PubMed
    1. Danckwerth F, Wuisman P, Ritter J, Blasius S, Jurgens H, Ozaki T, Winkelmann W. Osteosarkom bei zwei Geschwistern. Eine kasuistische Darstellung. Klin Padiatr. 1995;207:298–301. - PubMed
    1. Chauveinc L, Mosseri V, Quintana E, Desjardins L, Schlienger P, Doz F, Dutrillaux B. Osteosarcoma following retinoblastoma: age at onset and latency period. Ophthalmic Genet. 2001;22:77–88. - "V体育平台登录" PubMed
    1. Miller CW, Aslo A, Won A, Tan M, Lampkin B, Koeffler HP. Alterations of the p53, Rb and MDM2 genes in osteosarcoma. J Cancer Res Clin Oncol. 1996;122:559–565. - PubMed

Publication types (VSports最新版本)

MeSH terms